Age-related changes in myelin morphology, electrophysiological property and myelin-associated protein expression of mouse sciatic nerves
Age-related changes in myelin morphology, electrophysiological property and myelin-associated protein expression of mouse sciatic nerves
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小鼠坐骨神经髓磷脂形态、电生理特性和髓磷脂相关蛋白表达的年龄相关变化
DOI：
10.1016/j.neulet.2011.07.034
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发表时间：
2011-09
影响因子：
2.5
通讯作者：
Zhang, Qi
中科院分区：
文献类型：
--
作者：
Gu, Xiaosong;Shen, Dingding;Gao, Xiaorong;Ding, Fei;Zhang, Qi
本研究调查了周围神经在成熟和衰老过程中的形态和功能变化。在小鼠坐骨神经模型中，电子显微照片显示，髓鞘片层的数量从1周龄到12月龄逐渐增加，达到峰值，然后保持不变，直到18月龄；电生理检查显示，复合肌肉动作电位幅值从1周龄至18月龄逐渐增大，并与髓鞘片层数呈正线性相关。蛋白质印迹分析显示四种髓磷脂相关蛋白的年龄相关表达模式，即髓磷脂相关糖蛋白（MAG）、髓磷脂碱性蛋白（MBP）、糖蛋白P0（P0）和外周髓磷脂蛋白22（PMP22）。 MAG、MBP、PMP22与髓鞘片层数呈线性负相关或正相关。我们的研究结果表明，周围神经的形态和功能变化彼此密切相关，髓鞘相关蛋白对髓鞘的形成、成熟、变性和再生发挥不同的作用。
This study investigated the morphological and functional changes in peripheral nerves during the maturation and aging process. In a mouse sciatic nerve model, electron micrographs revealed that the number of myelin sheath lamellae gradually increased from 1 week through 12 months of age, when it reached the peak value, and then remained unchanged until 18 months of age; electrophysiological examinations showed that the amplitude of compound muscle action potentials gradually increased from 1 week through 18 months of age and displayed a positive linear correlation with the number of myelin sheath lamellae. Western blot analysis exhibited the age-related expression patterns of four myelin-associated proteins, i.e., myelin-associated glycoprotein (MAG), myelin basic protein (MBP), glycoprotein P0 (P0) and peripheral myelin protein 22 (PMP22). And MAG, MBP and PMP22 showed linear negative or positive correlations with the number of myelin sheath lamellae. Our results suggest that the morphological and functional changes in peripheral nerves are closely related to each other and the myelin-associated proteins perform distinct actions on the formation, maturation, degeneration and regeneration of myelin sheaths.
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影响因子：
4.2
作者：
Carina S. Johansson;M. Stenström;C. Hildebrand
通讯作者：
Carina S. Johansson;M. Stenström;C. Hildebrand
DOI：
10.1007/bf03160072
发表时间：
1993-12
期刊：
Molecular and chemical neuropathology
影响因子：
--
作者：
T. Inuzuka;S. Sato;N. Fujita;H. Baba;R. Nakano;H. Ishiguro;T. Miyatake
通讯作者：
T. Inuzuka;S. Sato;N. Fujita;H. Baba;R. Nakano;H. Ishiguro;T. Miyatake
影响因子：
2.9
作者：
Matsumoto, K;Ohnishi, K;Shimizu, Y
通讯作者：
Shimizu, Y
影响因子：
3.3
作者：
R. Melcangi;Valerio Magnaghi;Ilaria T. Cavarretta;Luciano Martini;F. Piva
通讯作者：
R. Melcangi;Valerio Magnaghi;Ilaria T. Cavarretta;Luciano Martini;F. Piva
影响因子：
2.9
作者：
Jeronimo, Andre;Dominques Jeronimo, Claudia Alem;Sassoli Fazan, Valeria Paula
通讯作者：
Sassoli Fazan, Valeria Paula