Customizable, wireless and implantable neural probe design and fabrication via 3D printing.
Customizable, wireless and implantable neural probe design and fabrication via 3D printing.
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DOI：
10.1038/s41596-022-00758-8
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发表时间：
2023-01
期刊：
影响因子：
14.8
通讯作者：
McCall, Jordan G.
中科院分区：
文献类型：
--
作者：
Parker, Kyle E.;Lee, Juhyun;Kim, Jenny R.;Kawakami, Chinatsu;Kim, Choong Yeon;Qazi, Raza;Jang, Kyung-In;Jeong, Jae-Woong;McCall, Jordan G.
该协议扩展描述了用于活体光遗传学的可快速定制的光学神经探针的低成本生产。我们详细介绍了使用3D打印机来制造微创的基于LED的无机神经探针，这种探针可以控制自由行为动物的神经电路活动，从而扩展了之前发表的两项协议的范围，该协议描述了用于研究完整神经系统的光电子器件的制造和实施。3D打印制造过程不需要广泛的培训，并且不需要昂贵的材料、专门的洁净室设施和传统制造过程中典型的耗时的微制造技术。因此，可以根据实验需要快速优化探头的设计，降低定制的成本和周转率。例如，3D打印的探针可以定制为针对多个大脑区域，也可以放大以用于大型动物模型。该方案包括三个步骤：1)探针制造，2)无线模块制备，3)体内分析的植入。对于经验丰富的研究人员来说，神经探头和无线模块的制造大约需要两天时间，而植入应该需要每只动物30-60分钟。行为分析所需的时间将根据实验设计的不同而有所不同，并应包括在植入探头之前至少5天的动物处理时间，以使每只动物熟悉它们的饲养员，从而减少可能影响行为分析结果的处理压力。定制探针的实施提高了光遗传学实验设计的灵活性，并增加了体内光遗传学研究使用无线探针的机会。
This Protocol Extension describes the low-cost production of rapidly customizable optical neural probes for in vivo optogenetics. We detail the use of a 3D printer to fabricate minimally invasive microscale inorganic LED-based neural probes which can control neural circuit activity in freely behaving animals, thus extending the scope of two previously published protocols describing the fabrication and implementation of optoelectronic devices for studying intact neural systems. The 3D-printing fabrication process does not require extensive training and eliminates the need for expensive materials, specialized cleanroom facilities, and time-consuming microfabrication techniques typical of conventional manufacturing processes. As a result, the design of the probes can be quickly optimized, based on experimental need, reducing the cost and turnaround for customization. For example, 3D-printed probes can be customized to target multiple brain regions or scaled up for use in large animal models. This protocol comprises three procedures: 1) probe fabrication, 2) wireless module preparation, and 3) implantation for in vivo assays. For experienced researchers, neural probe and wireless module fabrication requires approximately two days, while implantation should take 30–60 minutes per animal. Time required for behavioural assays will vary depending on the experimental design and should include at least 5 days of animal handling prior to the implantation of the probe, to familiarize each animal to their handler, thus reducing handling stress which may influence the result of the behavioural assays. The implementation of customized probes improves the flexibility in optogenetic experimental design and increases access to wireless probes for in vivo optogenetic research.
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影响因子：
48
作者：
Kim CK;Yang SJ;Pichamoorthy N;Young NP;Kauvar I;Jennings JH;Lerner TN;Berndt A;Lee SY;Ramakrishnan C;Davidson TJ;Inoue M;Bito H;Deisseroth K
通讯作者：
Deisseroth K
从杏仁核中的电路到行为。
影响因子：
64.8
作者：
Janak PH;Tye KM
通讯作者：
Tye KM
DOI：
10.1126/science.1232437
发表时间：
2013-04-12
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Kim TI;McCall JG;Jung YH;Huang X;Siuda ER;Li Y;Song J;Song YM;Pao HA;Kim RH;Lu C;Lee SD;Song IS;Shin G;Al-Hasani R;Kim S;Tan MP;Huang Y;Omenetto FG;Rogers JA;Bruchas MR
通讯作者：
Bruchas MR
影响因子：
19
作者：
Lee, Juhyun;Parker, Kyle E.;Jeong, Jae-Woong
通讯作者：
Jeong, Jae-Woong
影响因子：
7.9
作者：
Burton A;Won SM;Sohrabi AK;Stuart T;Amirhossein A;Kim JU;Park Y;Gabros A;Rogers JA;Vitale F;Richardson AG;Gutruf P
通讯作者：
Gutruf P