Photophysics of Cy3-encapsulated calcium phosphate nanoparticles.
Photophysics of Cy3-encapsulated calcium phosphate nanoparticles.
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DOI：
10.1021/nl803658w
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发表时间：
2009-04
期刊：
影响因子：
10.8
通讯作者：
Butler PJ
中科院分区：
文献类型：
--
作者：
Muddana HS;Morgan TT;Adair JH;Butler PJ
生物可降解的荧光磷酸钙（CP）纳米粒子作为一种生物成像剂的临床应用的进展需要详细的知识生色团与CP的相互作用。作为这种货物-基质相互作用的读数，我们使用稳态和时间分辨荧光光谱法确定了封装在CP纳米颗粒（CPNP）中的Cy 3的主要物理性质。荧光相关光谱法（FCS）确定的扩散系数和相关的流体动力学半径证实了高度单分散的CPNP的半径范围从7到10 nm的存在。单CP纳米颗粒比自由染料分子亮20倍，因为CP诱导的量子效率增加5倍，并且每个颗粒封装4个染料分子。当Cy 3被封装在CP中时，不存在由游离染料和溶剂之间的氢键或由溶剂粘度限制的分子内流动性引起的溶剂化变色位移。包封介导的辐射衰减率的增加和非辐射衰减率的降低，导致更长的荧光寿命的Cy 3是由于溶剂和CP相关的局部折射率和刚性CP的染料的灵活性受到限制。CPNP的增强的亮度使得能够使用标准和全内反射荧光（TIRF）模式在落射荧光下成像单个纳米颗粒，相机曝光时间为10毫秒的量级。这些增强的生物物理性质以及优异的生物相容性使CPNP成为从单分子跟踪到体内肿瘤检测的生物成像应用的理想选择，并提供定时共成像的可能性。控制细胞功能的药物。
Progress towards clinical application of biodegradable fluorescent calcium phosphate (CP) nanoparticles as a bioimaging agent requires detailed knowledge of chromophore interaction with CP. As readouts of this cargo-matrix interaction, we determined the principle photophysical properties of Cy3 encapsulated in CP nanparticles (CPNPs) using steady-state and time-resolved fluorescence spectroscopy. Fluorescence correlation spectroscopy (FCS)-determined diffusion coefficients and associated hydrodynamic radii confirmed the presence of highly monodisperse CPNPs with radii ranging from 7 to 10 nm. Single CP nanoparticles were 20 times brighter than free dye molecules because of a CP-induced 5-fold increase in quantum efficiency and encapsulation of 4 dye molecules per particle. Solvatochromic shifts resulting from hydrogen bonding between free dye and solvent or restricted intramolecular mobility by solvent viscosity were absent when Cy3 was encapsulated in CP. Encapsulation-mediated increases in radiative decay rates and decreases in non-radiative decay rates resulting in longer fluorescence lifetimes of Cy3 were attributed to solvent and CP-related local refractive indices and restricted flexibility of dye by rigid CP. Enhanced brightness of CPNPs enabled imaging of single nanoparticles under epi-fluorescence using both standard and total internal reflection fluorescence (TIRF) modes with camera exposure times on the order of 10s of ms. These enhanced photophysical properties together with excellent biocompatibility make CPNPs ideal for bioimaging applications ranging from single-molecule tracking to in vivo tumor detection and offer the possibility of timed co-delivery of drugs to control cell function.
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影响因子：
10.8
作者：
Kester M;Heakal Y;Fox T;Sharma A;Robertson GP;Morgan TT;Altinoğlu EI;Tabaković A;Parette MR;Rouse SM;Ruiz-Velasco V;Adair JH
通讯作者：
Adair JH
影响因子：
2.9
作者：
Jia, Ke;Wan, Yan;Yang, Guoqiang
通讯作者：
Yang, Guoqiang
影响因子：
29.4
作者：
Barbé, C;Bartlett, J;Calleja, G
通讯作者：
Calleja, G
影响因子：
56.9
作者：
Dubertret, B;Skourides, P;Libchaber, A
通讯作者：
Libchaber, A
DOI：
10.1128/cdli.7.6.899-903.2000
发表时间：
2000-11-01
期刊：
CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY
影响因子：
--
作者：
He, Q;Mitchell, AR;Bell, SJD
通讯作者：
Bell, SJD