Spatial structural integrity is important for adipose regeneration after transplantation
Spatial structural integrity is important for adipose regeneration after transplantation
复制标题
空间结构完整性对于移植后脂肪再生很重要
DOI：
10.1007/s00403-015-1574-y
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发表时间：
2015-05
影响因子：
3
通讯作者：
Lu Feng
中科院分区：
文献类型：
--
作者：
Yuan Yi;Zhang Shu;Gao Jianhua;Lu Feng
结构脂肪移植技术的进步显著提高了移植物的存活率和稳定性。本研究探讨了脂肪组织空间结构完整性对脂肪移植后脂肪再生的重要性。我们试图加强对结构性脂肪移植的理解，并优化用于脂肪组织的临床获取、纯化和移植的程序。在−20℃冷冻3天建立的结构失活脂肪组织模型中，细胞几乎全部死亡，但结构完好。我们将该脂肪组织模型（A组）或未处理的脂肪组织（B组）移植到表达gfp的小鼠体内。B组移植物成活率高于a组，纤维化程度低于a组。a组巨噬细胞浸润（F4/80）峰值时间较B组长，两组血管密度（CD31）变化相似，在移植后4周达到峰值，随后下降。在两组中，Ki67+细胞的数量表现出相似的趋势。与B组相比，A组在移植后4-8周有更多的Ki67+细胞，但在移植后12周这些细胞较少。脂肪组织在脂肪细胞和细胞外基质的支持下，其完整的空间结构为脂肪移植后的脂肪生成和血管生成提供了生态位。
Advances in structural fat transplantation technology have significantly improved the survival rate and stability of grafts. This study investigated the importance of the spatial structural integrity of adipose tissue for adipose regeneration after fat transplantation. We sought to enhance understanding of structural fat transplantation and optimize procedures used for the clinical acquisition, purification, and transplantation of adipose tissue. In an inactivated structuration adipose tissue model established by freezing at −20 °C for 3 days, nearly all cells were dead but the structure was intact. We transplanted this adipose tissue model (group A) or non-treated adipose tissue (group B) into GFP-expressing mice. Group B showed a higher graft survival percentage and less fibrosis than group A. The macrophage infiltration (F4/80) peak period was longer in group A than in group B. The change in vessel density (CD31) was similar in the two groups: it peaked at 4 weeks after transplantation and decreased thereafter. In both groups, the number of Ki67+ cells showed a similar trend. In comparison to group B, group A had more Ki67+ cells at 4–8 weeks after transplantation, but fewer of these cells at 12 weeks after transplantation. The intact spatial structure of adipose tissue, which is supported by adipocytes and extracellular matrix, provides a niche for adipogenesis and angiogenesis after fat transplantation.
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影响因子：
64.5
作者：
Scadden DT
通讯作者：
Scadden DT
影响因子：
4
作者：
Yang, Kimberline R.;Mooney, Steven M.;Zarif, Jelani C.;Coffey, Donald S.;Taichman, Russell S.;Pienta, Kenneth J.
通讯作者：
Pienta, Kenneth J.
影响因子：
2.9
作者：
Pu, Lee L Q;Cui, Xiangdong;Gao, Dayong
通讯作者：
Gao, Dayong
影响因子：
2.5
作者：
Chiu, Yu-Chieh;Cheng, Ming-Huei;Brey, Eric M.
通讯作者：
Brey, Eric M.
影响因子：
3.6
作者：
Wu I;Nahas Z;Kimmerling KA;Rosson GD;Elisseeff JH
通讯作者：
Elisseeff JH