Identification and characterization of intervening sequences within 23S rRNA genes from more than 200 Campylobacter isolates from seven species including atypical campylobacters.
Identification and characterization of intervening sequences within 23S rRNA genes from more than 200 Campylobacter isolates from seven species including atypical campylobacters.
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DOI：
10.1186/1471-2180-9-256
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发表时间：
2009-12-11
期刊：
影响因子：
4.2
通讯作者：
Matsuda M
中科院分区：
文献类型：
--
作者：
Tazumi A;Kakinuma Y;Misawa N;Moore JE;Millar BC;Matsuda M
采用两对PCR引物对弯曲菌（包括非典型弯曲菌）23S rRNA基因的中间序列（IVSs）进行了鉴定和鉴定，设计了螺旋25区和45区。只有c sputorum生物变型sputorum LMG7975和fecalis LMG8531, LMG8534和LMG6728 204弯曲杆菌分离株(n = 56 c .空肠;n = 11 c .杆菌;n = 33 c .胎儿;n = 43海;n = 30 c . hyointestinalis; n = 4 c sputorum生物变型sputorum; n = 5 c sputorum生物变型fecalis; n = 5 c sputorum生物变型paraureolyticus; n = 10 c . concisus; n = 7 c . curvus)被显示在螺旋25个地区进行静脉注射。有趣的是，粪孢C. sputorum biovar fecalis LMG8531和LMG8534分别携带了两种不同的23S rRNA基因。因此，在269个分离株的265个分离株中，包括之前检测的65个lari分离株，在螺旋25区发现了IVSs的缺失。在螺旋45区，所有hyotestinalis、C. sputorum和C. concisus分离株均未携带任何ivs。56株C. jejuni中有30株（54%）、11株C. coli中有5株（45%）、33株C.胎儿中有25株（76%）、43株C. upsaliensis中有30株（70%）、7株C. curvus中有6株（90%）携带ivs。在C. jejuni和C. upsaliensis分离株中，鉴定出两种不同的23S rRNA基因分别在螺旋45区有ivs和没有ivs时出现。用本研究中鉴定的所有IVSs构建二级结构模型。在携带16S和23S rRNA基因的分离株纯化的RNA中，分别没有16S和23S rRNA。
Identification and characterization of intervening sequences (IVSs) within 23S rRNA genes from Campylobacter organisms including atypical campylobacters were carried out using two PCR primer pairs, designed to generate helix 25 and 45 regions. Only C. sputorum biovar sputorum LMG7975 and fecalis LMG8531, LMG8534 and LMG6728 of a total of 204 Campylobacter isolates (n = 56 C. jejuni; n = 11 C. coli; n = 33 C. fetus; n = 43 C. upsaliensis; n = 30 C. hyointestinalis; n = 4 C. sputorum biovar sputorum; n = 5 C. sputorum biovar fecalis; n = 5 C. sputorum biovar paraureolyticus; n = 10 C. concisus; n = 7 C. curvus) were shown to carry IVSs in helix 25 region. C. sputorum biovar fecalis LMG8531 and LMG8534, interestingly, carried two different kinds of the 23S rRNA genes with and without the IVS, respectively. Consequently, in a total of 265 isolates of 269, including 65 C. lari isolates examined previously, the absence of IVSs was identified in the helix 25 region. In the helix 45 region, all the C. hyointestinalis, C. sputorum and C. concisus isolates were shown not to carry any IVSs. However, the 30 of 56 C. jejuni isolates (54%), 5 of 11 C. coli (45%), 25 of 33 C. fetus (76%), 30 of 43 C. upsaliensis (70%) and 6 of 7 C. curvus (90%) were shown to carry IVSs. In C. jejuni and C. upsaliensis isolates, two different kinds of the 23S rRNA genes were also identified to occur with and without IVSs in the helix 45 region, respectively. Secondary structure models were also constructed with all the IVSs identified in the present study. In the purified RNA fractions from the isolates which carried the 16S or 23S rRNA genes with the IVSs, no 16S or 23S rRNA was evident, respectively.
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影响因子：
3.2
作者：
Pronk, LM;Sanderson, KE
通讯作者：
Sanderson, KE
DOI：
10.1099/ijs.0.000851-0
发表时间：
2009-05-01
影响因子：
2.8
作者：
Debruyne, Lies;On, Stephen L. W.;Vandamme, Peter
通讯作者：
Vandamme, Peter
影响因子：
9.4
作者：
Lawson, AJ;Logan, JMJ;Stanley, J
通讯作者：
Stanley, J
影响因子：
2.8
作者：
Miller, William G.;Wang, Guilin;Parker, Craig T.
通讯作者：
Parker, Craig T.
影响因子：
3.1
作者：
Tazumi, Akihiro;Kakinuma, Yuki;Matsuda, Motoo
通讯作者：
Matsuda, Motoo