Fabrication, characterization and cellular compatibility of poly(hydroxy alkanoate) composite nanofibrous scaffolds for nerve tissue engineering.
Fabrication, characterization and cellular compatibility of poly(hydroxy alkanoate) composite nanofibrous scaffolds for nerve tissue engineering.
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DOI：
10.1371/journal.pone.0057157
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Moroni L
中科院分区：
文献类型：
--
作者：
Masaeli E;Morshed M;Nasr-Esfahani MH;Sadri S;Hilderink J;van Apeldoorn A;van Blitterswijk CA;Moroni L
利用聚合物支架和细胞相结合的组织工程技术代表了一种有前途的神经再生方法。我们通过将聚（3-羟基丁酸酯）（PHB）和聚（3-羟基丁酸酯-co-3-羟基戊酸酯）（PHBV）以不同的组合物共混来制备静电纺丝支架，以研究它们对髓鞘膜再生的潜力。采用差示扫描量热法（DSC）对纳米纤维共混物的热性能进行了分析，结果表明，随着PHBV质量比的增加，共混物的熔融温度、玻璃化温度和结晶度均降低。拉曼光谱还显示，以1725 cm-1为中心的谱带的半高全宽可用于估计电纺网的结晶度。随机和排列的纳米纤维支架也通过静电纺丝的聚羟基丁酸酯和PHBV与或不与I型胶原蛋白。研究了共混物组成、纤维排列和胶原蛋白掺入对雪旺细胞（SCs）组织和功能的影响。SC在所有支架制剂上附着和增殖长达14天。在定向的PHB/PHBV/胶原纤维上生长的SC表现出沿着纤维方向取向的双极形态，而在随机取向的纤维上生长的SC具有多极形态。在纳米纤维中掺入胶原蛋白在第14天增加了SC增殖，在第7天增加了GDNF基因表达，在第6天增加了NGF分泌。本研究的结果表明，对齐的聚羟基丁酸/聚羟基丁酸乙脂静电纺丝纳米纤维可以找到潜在的用途作为神经组织工程应用的支架，并在纳米纤维中的I型胶原蛋白的存在下，提高细胞分化。
Tissue engineering techniques using a combination of polymeric scaffolds and cells represent a promising approach for nerve regeneration. We fabricated electrospun scaffolds by blending of Poly (3-hydroxybutyrate) (PHB) and Poly (3-hydroxy butyrate-co-3- hydroxyvalerate) (PHBV) in different compositions in order to investigate their potential for the regeneration of the myelinic membrane. The thermal properties of the nanofibrous blends was analyzed by differential scanning calorimetry (DSC), which indicated that the melting and glass temperatures, and crystallization degree of the blends decreased as the PHBV weight ratio increased. Raman spectroscopy also revealed that the full width at half height of the band centered at 1725 cm−1 can be used to estimate the crystalline degree of the electrospun meshes. Random and aligned nanofibrous scaffolds were also fabricated by electrospinning of PHB and PHBV with or without type I collagen. The influence of blend composition, fiber alignment and collagen incorporation on Schwann cell (SCs) organization and function was investigated. SCs attached and proliferated over all scaffolds formulations up to 14 days. SCs grown on aligned PHB/PHBV/collagen fibers exhibited a bipolar morphology that oriented along the fiber direction, while SCs grown on the randomly oriented fibers had a multipolar morphology. Incorporation of collagen within nanofibers increased SCs proliferation on day 14, GDNF gene expression on day 7 and NGF secretion on day 6. The results of this study demonstrate that aligned PHB/PHBV electrospun nanofibers could find potential use as scaffolds for nerve tissue engineering applications and that the presence of type I collagen in the nanofibers improves cell differentiation.
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