Myocardial interstitial Cajal-like cells (ICLC) and their nanostructural relationships with intercalated discs: shed vesicles as intermediates.
Myocardial interstitial Cajal-like cells (ICLC) and their nanostructural relationships with intercalated discs: shed vesicles as intermediates.
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DOI：
10.1111/j.1582-4934.2007.00117.x
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发表时间：
2007-09
影响因子：
5.3
通讯作者：
Gherghiceanu M
中科院分区：
文献类型：
--
作者：
Mandache E;Popescu LM;Gherghiceanu M
间质盘（Intercalated discs，ID）是机械和电化学连接心肌细胞的复杂连接单位。然而，关于心肌细胞与间质性非肌细胞相互作用的信息有限。我们前期的研究表明，心肌间质Cajal样细胞（ICLC）位于心肌细胞、毛细血管和神经纤维之间。典型地，ICLC具有几个非常长的念珠状细胞质突起，其与神经纤维以及彼此建立紧密接触。我们在这里报告的ICLC过程与ID的关系的超微结构证据。ICLC细胞质的膨胀（几十微米长）优先通过或停止附近的ID。透射电子显微镜强调了三个不同的连接功能之间的提示ICLC的扩展和肌细胞在“口”的ID：自由或出芽脱落囊泡，胞吐多囊泡体和直接接触。在最后一种情况下，电子致密的重复纳米结构（“柱”）（35-40 nm高，100-150 nm宽，类似于粘附分子）将ICLC固定在肌细胞上。所有这些特征表明，在ID的微环境中，ICLC和心肌细胞之间存在一种共同的和/或旁分泌的相互调节，可能监测心脏功能，特别是电活动。
Intercalated discs (ID) are complex junctional units that connect cardiac myocytes mechanically and electrochemically. However, there is limited information concerning the cardiomyocyte interaction with interstitial non-muscle cells. Our previous studies showed that myocardial interstitial Cajal-like cells (ICLC) are located in between cardiomyocytes, blood capillaries and nerve fibres. Typically, ICLC have several very long, moniliform, cytoplasmic processes which establish closed contacts with nerve fibres, as well as each other. We report here ultrastructural evidence concerning the relationships of ICLC processes with ID. The ICLC cytoplasmic prolongations (tens micrometers length) preferentially pass by or stop nearby the ID. Transmission electron microscopy emphasized three distinct connecting features between the tips of ICLC extensions and myocytes at the ‘mouth’ of ID: free or budding shed vesicles, exocytotic multi-vesicular bodies and direct contacts. In the last case, electron-dense repetitive nanostructures (‘pillars’) (35–40 nm high and 100–150 nm wide, similar to adhesion molecules) fasten the ICLC to the myocytes. All these features suggest a juxtacrine and/or paracrine intercellular mutual modulation of ICLC and cardiomyocytes in the microenvironment of ID, possibly monitoring the cardiac functions, particularly the electrical activity.
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影响因子：
4.5
作者：
Lyons, Alan D.;Gardiner, Tom A.;McCloskey, Karen D.
通讯作者：
McCloskey, Karen D.
DOI：
10.1111/j.1582-4934.2006.tb00306.x
发表时间：
2006-01
影响因子：
5.3
作者：
Hinescu ME;Gherghiceanu M;Mandache E;Ciontea SM;Popescu LM
通讯作者：
Popescu LM
影响因子：
3.2
作者：
Hinescu, Mihail E.;Ardeleanu, Carmen;Popescu, Laurentziu M.
通讯作者：
Popescu, Laurentziu M.
影响因子：
3.2
作者：
Erlandsen, SL;Bittermann, AG;Marko, M
通讯作者：
Marko, M
影响因子：
5.3
作者：
Junquera C;Martínez-Ciriano C;Castiella T;Serrano P;Azanza MJ;Junquera SR
通讯作者：
Junquera SR