Functional Characterization and Signaling Systems of Corazonin and Red Pigment Concentrating Hormone in the Green Shore Crab, Carcinus maenas.
Functional Characterization and Signaling Systems of Corazonin and Red Pigment Concentrating Hormone in the Green Shore Crab, Carcinus maenas.
复制标题
绿海岸蟹Carcinus maenas中corazonin和红色色素浓缩激素的功能表征和信号传导系统。
DOI：
10.3389/fnins.2017.00752
复制
发表时间：
2017
影响因子：
4.3
通讯作者：
Webster SG
中科院分区：
文献类型：
--
作者：
Alexander JL;Oliphant A;Wilcockson DC;Audsley N;Down RE;Lafont R;Webster SG
神经肽作为神经递质、神经调质和激素在节肢动物的生理活动中起着重要作用。后基因组激增确定的神经肽及其推定的受体还没有匹配的配体-受体对的功能表征。事实上，直到最近，没有G蛋白偶联受体（GPCRs）已在任何甲壳类动物的功能定义。在这里，我们探讨结构相关的，功能多样的促性腺激素释放激素旁系同源物，corazonin（CRZ）和红色素浓缩激素（RPCH）和它们的G蛋白偶联受体（GPCRs）在螃蟹，巨蟹。使用水母发光蛋白发光来测量体外Ca 2+动员，我们证明了CRZ和RPCH的受体-配体配对。CRZR激活的细胞信号以剂量依赖性方式（EC 50 0.75 nM）和与昆虫CRZ肽的比较研究表明，这种肽的C-末端在受体-配体相互作用中很重要。RPCH与RPCHR的相互作用具有极高的灵敏度（EC 50 20 pM）。既没有受体结合GnRH，也没有AKH/CRZ相关肽。两种受体的转录物分布表明，CRZR表达出乎意料地限于Y器官（YO）。CRZ肽对YO的应用对蜕皮激素的生物合成没有影响，除了在蜕皮后早期有适度的刺激。CRZ对心脏活动、血糖水平、脂质动员或色素细胞中的色素分布没有影响，这一情况反映了其mRNA的分布。除了众所周知的RPCH作为促色素激素的活性外，它还间接引起高血糖症（这是眼柄依赖性的）。RPCHR mRNA也在卵巢中表达，表明可能在生殖中的作用。CRZ和RPCH神经元在神经系统中的解剖详细描述了免疫组织化学和原位杂交。每种肽在CNS中具有广泛但不重叠的分布，神经解剖学表明两者都可能从连合后器官释放。这项研究是第一个在甲壳类动物中去除GPCR的，并为这些进化相关的神经肽的迄今未知和多样的功能提供证据。
Neuropeptides play a central role as neurotransmitters, neuromodulators and hormones in orchestrating arthropod physiology. The post-genomic surge in identified neuropeptides and their putative receptors has not been matched by functional characterization of ligand-receptor pairs. Indeed, until very recently no G protein-coupled receptors (GPCRs) had been functionally defined in any crustacean. Here we explore the structurally-related, functionally-diverse gonadotropin-releasing hormone paralogs, corazonin (CRZ) and red-pigment concentrating hormone (RPCH) and their G-protein coupled receptors (GPCRs) in the crab, Carcinus maenas. Using aequorin luminescence to measure in vitro Ca2+ mobilization we demonstrated receptor-ligand pairings of CRZ and RPCH. CRZR-activated cell signaling in a dose-dependent manner (EC50 0.75 nM) and comparative studies with insect CRZ peptides suggest that the C-terminus of this peptide is important in receptor-ligand interaction. RPCH interacted with RPCHR with extremely high sensitivity (EC50 20 pM). Neither receptor bound GnRH, nor the AKH/CRZ-related peptide. Transcript distributions of both receptors indicate that CRZR expression was, unexpectedly, restricted to the Y-organs (YO). Application of CRZ peptide to YO had no effect on ecdysteroid biosynthesis, excepting a modest stimulation in early post-molt. CRZ had no effect on heart activity, blood glucose levels, lipid mobilization or pigment distribution in chromatophores, a scenario that reflected the distribution of its mRNA. Apart from the well-known activity of RPCH as a chromatophorotropin, it also indirectly elicited hyperglycemia (which was eyestalk-dependent). RPCHR mRNA was also expressed in the ovary, indicating possible roles in reproduction. The anatomy of CRZ and RPCH neurons in the nervous system is described in detail by immunohistochemistry and in situ hybridization. Each peptide has extensive but non-overlapping distribution in the CNS, and neuroanatomy suggests that both are possibly released from the post-commissural organs. This study is one of the first to deorphanize a GPCR in a crustacean and to provide evidence for hitherto unknown and diverse functions of these evolutionarily-related neuropeptides.
登录
查看更多内容
影响因子：
5.2
作者：
Caers J;Verlinden H;Zels S;Vandersmissen HP;Vuerinckx K;Schoofs L
通讯作者：
Schoofs L
影响因子：
4.6
作者：
Chung, JS;Webster, SG
通讯作者：
Webster, SG
DOI：
10.1016/j.bbrc.2006.03.117
发表时间：
2006-05-26
影响因子：
3.1
作者：
Belmont, M;Cazzamali, G;Grimmelikhuijzen, CJP
通讯作者：
Grimmelikhuijzen, CJP
DOI：
10.1016/s0006-291x(02)02398-7
发表时间：
2002-10-18
影响因子：
3.1
作者：
Cazzamali, G;Saxild, NPE;Grimmelikhuijzen, CJP
通讯作者：
Grimmelikhuijzen, CJP
影响因子：
3.7
作者：
Christie AE;Chi M;Lameyer TJ;Pascual MG;Shea DN;Stanhope ME;Schulz DJ;Dickinson PS
通讯作者：
Dickinson PS