Inactivation Kinetics of G-Quadruplex/Hemin Complex and Optimization for More Reliable Catalysis.
Inactivation Kinetics of G-Quadruplex/Hemin Complex and Optimization for More Reliable Catalysis.
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g -四联体/血红蛋白配合物失活动力学及更可靠的催化优化。
DOI：
10.1002/cplu.202200090
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发表时间：
2022-07
期刊：
影响因子：
3.4
通讯作者：
Fu, Jinglin
中科院分区：
文献类型：
--
作者：
Monte Carlo, Anthony R., III;Fu, Jinglin
可靠的催化对于各种化学品的合成、分子传感和生物医学至关重要。G-quadruplex/Hemin（GQH）复合物是一种具有过氧化物酶活性的DNA酶，已被广泛应用于各种出版物中。然而，一个关注存在的GQH催化过氧化反应的不稳定动力学。本工作研究了导致GQH失活和信号降解的几个因素，包括pH、缓冲液组分、底物的选择和辅因子的氧化损伤。使用比色和荧光测定，发现GQH在碱性条件下高度不稳定，在高H2 O2浓度下2分钟内失去50%的GQH活性。适当的条件和底物，建议准确表征GQH催化的反应，以及优化，以提高催化的可靠性，如使用多组氨酸和级联反应。这些结果可能是有用的GQH相关的应用。
Reliable catalysis is critical for the synthesis of various chemicals, molecular sensing and biomedicine. G-quadruplex/Hemin (GQH) complex, a peroxidase-mimicking DNAzyme, has been widely used in various publications. However, a concern exists about the unstable kinetics of GQH-catalyzed peroxidation. This work investigates several factors that result in the inactivation of GQH and the signal degradation during long reaction periods, including pH, buffer component, the selection of substrate and the oxidation damage of cofactor. Using colorimetric and fluorescent assays, GQH was found to be highly unstable under basic conditions with 50% of GQH activity lost within 2 minutes at high H2O2 concentrations. Appropriate conditions and substrates are suggested for accurately characterizing GQH-catalyzed reactions, as well as optimization to improve the catalytic reliability, such as the use of polyhistidine and cascade reactions. These results could be useful for GQH-related applications.
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影响因子：
3.8
作者：
Veitch, NC
通讯作者：
Veitch, NC
影响因子：
13.3
作者：
Fu, Jinglin;Oh, Sung Won;Zhang, Ting
通讯作者：
Zhang, Ting
影响因子：
17.1
作者：
Liu, Qing;Wang, Hui;Ding, Baoquan
通讯作者：
Ding, Baoquan
影响因子：
46.2
作者：
通讯作者：
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影响因子：
17.1
作者：
Atsumi, Hiroshi;Belcher, Angela M.
通讯作者：
Belcher, Angela M.