Social motility of biofilm-like microcolonies in a gliding bacterium.
Social motility of biofilm-like microcolonies in a gliding bacterium.
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DOI：
10.1038/s41467-021-25408-7
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发表时间：
2021-09-29
影响因子：
16.6
通讯作者：
Beebe DJ
中科院分区：
文献类型：
--
作者：
Li C;Hurley A;Hu W;Warrick JW;Lozano GL;Ayuso JM;Pan W;Handelsman J;Beebe DJ
细菌生物膜是包埋在胞外多糖（EPS）基质中的表面相关细胞的聚集体，并且通常是静止的。对细菌集体运动的研究主要集中在没有生物膜的情况下由鞭毛或皮利介导的群集运动。在这里，我们描述了一种独特的集体运动模式的自我推进，表面相关的生物膜样多细胞结构。在油下开放微流控系统中观察到，约翰逊黄杆菌细胞通过滑动运动自组装成具有EPS核心的球形小菌落。小的菌落合并，产生更大的菌落。显微镜分析和计算机模拟表明，小菌落移动的细胞在该结构的基础上，连接到表面的细胞的一个极点。生化和突变分析表明，一个积极的过程驱动小菌落的自组装和运动，这取决于细菌的滑行装置。我们假设，这种模式的集体细菌运动固体表面上可能发挥潜在的作用，生物膜动力学，细菌货物运输，或微生物的适应。然而，无论这种集体运动是发生在植物根系还是土壤颗粒上，F。约翰逊是未知的。细菌生物膜是细菌表面附着的细胞在胞外多糖（EPS）基质中的聚集体。在这里，作者描述了一种独特的集体运动模式，由自推进，表面相关的球形小菌落与EPS核心的滑行细菌黄杆菌约翰逊。
Bacterial biofilms are aggregates of surface-associated cells embedded in an extracellular polysaccharide (EPS) matrix, and are typically stationary. Studies of bacterial collective movement have largely focused on swarming motility mediated by flagella or pili, in the absence of a biofilm. Here, we describe a unique mode of collective movement by a self-propelled, surface-associated biofilm-like multicellular structure. Flavobacterium johnsoniae cells, which move by gliding motility, self-assemble into spherical microcolonies with EPS cores when observed by an under-oil open microfluidic system. Small microcolonies merge, creating larger ones. Microscopic analysis and computer simulation indicate that microcolonies move by cells at the base of the structure, attached to the surface by one pole of the cell. Biochemical and mutant analyses show that an active process drives microcolony self-assembly and motility, which depend on the bacterial gliding apparatus. We hypothesize that this mode of collective bacterial movement on solid surfaces may play potential roles in biofilm dynamics, bacterial cargo transport, or microbial adaptation. However, whether this collective motility occurs on plant roots or soil particles, the native environment for F. johnsoniae, is unknown. Bacterial biofilms are aggregates of surface-associated cells embedded in an extracellular polysaccharide (EPS) matrix. Here, the authors describe a unique mode of collective movement by self-propelled, surface-associated spherical microcolonies with EPS cores in the gliding bacterium Flavobacterium johnsoniae.
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影响因子：
6.1
作者：
Li C;Yu J;Paine P;Juang DS;Berry SM;Beebe DJ
通讯作者：
Beebe DJ
影响因子：
56.9
作者：
Chamot-Rooke, Julia;Mikaty, Guillain;Dumenil, Guillaume
通讯作者：
Dumenil, Guillaume
影响因子：
3.6
作者：
O'Toole, GA;Kolter, R
通讯作者：
Kolter, R
影响因子：
9.5
作者：
Li, Chao;Yu, Jiaquan;Beebe, David J.
通讯作者：
Beebe, David J.
影响因子：
3.2
作者：
Hunnicutt, DW;McBride, MJ
通讯作者：
McBride, MJ