Analysis of commonly expressed genes between first trimester fetal heart and placenta cell types in the context of congenital heart disease.
Analysis of commonly expressed genes between first trimester fetal heart and placenta cell types in the context of congenital heart disease.
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DOI：
10.1038/s41598-022-14955-8
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发表时间：
2022-06-24
影响因子：
4.6
通讯作者：
Jones, Helen N.
中科院分区：
文献类型：
--
作者：
Wilson, Rebecca L.;Yuan, Victor;Courtney, Jennifer A.;Tipler, Alyssa;Cnota, James F.;Jones, Helen N.
先天性心脏病（CHD）通常与胎儿生长异常有关。在怀孕的前三个月，心脏和胎盘同时发育，并共享关键的发育途径。据推测，任一器官的形态发生缺陷是协同相关的。然而，许多致力于了解先心病背后机制的研究忽视了胎盘的贡献。在这项研究中，我们的目的是使用两个公开的单细胞测序数据库来识别妊娠早期心脏和胎盘细胞之间常见表达的基因。使用系统计算方法，我们鉴定了心脏和胎盘内皮细胞之间的 328 个常见表达基因，以及血管发育（GO：0001944，FDR 2.90E−30）和血管生成（GO：0001525，FDR 1.18E−27）等途径的富集。我们还发现，与胎儿心脏内皮细胞相比，胎盘绒毛外滋养层共有197个表达基因，细胞滋养层共有128个表达基因，合体滋养层共有80个表达基因，包括FLT1、GATA2、ENG和CDH5等基因。最后，妊娠早期心肌细胞和胎盘细胞滋养层的比较揭示了 53 个常见表达基因和细胞功能不可或缺的生物过程的富集，包括细胞呼吸 (GO:0045333; FDR 5.05E−08)、离子转运 (GO:0006811; FDR 2.08E−02) 和氧化还原过程 (GO:0055114; FDR) 1.58E−07)。总体而言，我们的结果确定了妊娠早期胎儿心脏和胎盘细胞之间常见的特定基因和细胞途径，如果这些基因和细胞途径受到破坏，可能会同时导致发育紊乱，从而导致冠心病。
Congenital heart disease (CHD) is often associated with fetal growth abnormalities. During the first trimester of pregnancy, the heart and placenta develop concurrently, and share key developmental pathways. It is hypothesized that defective morphogenesis of either organ is synergistically linked. However, many studies determined to understand the mechanisms behind CHD overlook the contribution of the placenta. In this study, we aimed to identify commonly expressed genes between first trimester heart and placenta cells using two publicly available single cell sequencing databases. Using a systematic computational approach, we identified 328 commonly expressed genes between heart and placenta endothelial cells and enrichment in pathways including Vasculature Development (GO:0001944, FDR 2.90E−30), and Angiogenesis (GO:0001525, FDR 1.18E−27). We also found, in comparison with fetal heart endothelial cells, 197 commonly expressed genes with placenta extravillous trophoblasts, 128 with cytotrophoblasts and 80 with syncytiotrophoblasts, and included genes such as FLT1, GATA2, ENG and CDH5. Finally, comparison of first trimester cardiomyocytes and placenta cytotrophoblasts revealed 53 commonly expressed genes and enrichment in biological processes integral to cellular function including Cellular Respiration (GO:0045333; FDR 5.05E−08), Ion Transport (GO:0006811; FDR 2.08E−02), and Oxidation–Reduction Process (GO:0055114; FDR 1.58E−07). Overall, our results identify specific genes and cellular pathways common between first trimester fetal heart and placenta cells which if disrupted may concurrently contribute to the developmental perturbations resulting in CHD.
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影响因子：
3.8
作者：
Aplin, JD;Straszewski-Chavez, SL;Knöfler, M
通讯作者：
Knöfler, M
DOI：
10.1016/j.ejogrb.2005.07.006
发表时间：
2006-04-01
影响因子：
2.6
作者：
Cedergren, MI;Källén, BAJ
通讯作者：
Källén, BAJ
影响因子：
8.8
作者：
Cui, Yueli;Zheng, Yuxuan;Tang, Fuchou
通讯作者：
Tang, Fuchou
DOI：
10.1002/ajmg.1535.abs
发表时间：
2001-10-15
期刊：
AMERICAN JOURNAL OF MEDICAL GENETICS
影响因子：
--
作者：
Kobashi, G;Yamada, H;Fujimoto, S
通讯作者：
Fujimoto, S
影响因子：
4
作者：
Courtney JA;Cnota JF;Jones HN
通讯作者：
Jones HN