Effect of interleukin-1β on occludin mRNA expression in the duodenal and colonic mucosa of dogs with inflammatory bowel disease.
Effect of interleukin-1β on occludin mRNA expression in the duodenal and colonic mucosa of dogs with inflammatory bowel disease.
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DOI：
10.1111/jvim.15117
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发表时间：
2018-05
影响因子：
2.6
通讯作者：
Ohmori K
中科院分区：
文献类型：
--
作者：
Ogawa M;Osada H;Hasegawa A;Ohno H;Yanuma N;Sasaki K;Shimoda M;Shirai J;Kondo H;Ohmori K
据报道，在患有炎症性肠病（IBD）的犬的十二指肠粘膜中存在白细胞介素（IL）-1 β和IL-1受体拮抗剂（Ra）的粘液失衡。然而，结肠粘膜的不平衡及其在犬IBD的肠炎和结肠炎中的作用仍不清楚。测定IBD犬结肠粘膜中IL-1β和IL-1 Ra蛋白的表达，并确定IL-1β对IBD犬十二指肠和结肠粘膜中紧密连接组分occludin（ocln）mRNA表达的影响。12只IBD犬和6只健康犬。在12只IBD犬中的7只中，通过ELISA定量结肠粘膜中的IL-1β和IL-1 Ra蛋白。通过真实的时间PCR检测12只犬的十二指肠和结肠粘膜中ocln mRNA的表达。IBD犬结肠粘膜中IL-1β与IL-1 Ra的比值显著高于健康犬。离体实验确定IL-1β抑制健康犬结肠粘膜中ocln mRNA的表达，但不抑制十二指肠粘膜中ocln mRNA的表达。ocln mRNA的表达在结肠粘膜，但不是在十二指肠粘膜，在IBD的狗比健康狗显着降低。IL-1β的相对增加可能会减弱ocln的表达，导致肠屏障功能障碍，并促进IBD犬结肠粘膜的肠道炎症，但不会促进IBD犬十二指肠粘膜的肠道炎症。
Mucosal imbalance of interleukin (IL)‐1β and IL‐1 receptor antagonist (Ra) has been reported in the duodenal mucosa of dogs with inflammatory bowel disease (IBD). However, the imbalance in the colonic mucosa and its role in duodenitis and colitis in IBD of dogs remain unclear. To measure the expression of IL‐1β and IL‐1Ra proteins in the colonic mucosa of dogs with IBD, and to determine the effect of IL‐1β on expression of occludin (ocln) mRNA, a tight junction component, in the duodenal and colonic mucosa of dogs with IBD. Twelve dogs with IBD and 6 healthy dogs. IL‐1β and IL‐1 Ra proteins in the colonic mucosa were quantified by ELISA in 7 of the 12 dogs with IBD. Expression of ocln mRNA in the duodenal and colonic mucosa was examined in the 12 dogs by real‐time PCR. The ratio of IL‐1β to IL‐1Ra in the colonic mucosa was significantly higher in dogs with IBD than in healthy dogs. The ex vivo experiment determined that IL‐1β suppressed expression of ocln mRNA in the colonic mucosa, but not in the duodenal mucosa, of healthy dogs. Expression of ocln mRNA in the colonic mucosa, but not in the duodenal mucosa, was significantly lower in dogs with IBD than in healthy dogs. A relative increase in IL‐1β may attenuate ocln expression, leading to intestinal barrier dysfunction and promotion of intestinal inflammation in the colonic mucosa, but not in the duodenal mucosa, of dogs with IBD.
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DOI：
10.1038/nrmicro3552
发表时间：
2016-01
期刊：
Nature reviews. Microbiology
影响因子：
--
作者：
Donaldson GP;Lee SM;Mazmanian SK
通讯作者：
Mazmanian SK
影响因子：
2.4
作者：
Jergens, A. E.;Evans, R. B.;Day, M. J.
通讯作者：
Day, M. J.
影响因子：
2.6
作者：
Procoli, F.;Motskuela, P. F.;Allenspach, K.
通讯作者：
Allenspach, K.
影响因子：
2.6
作者：
Garosi, L;McConnell, JF;Schatzberg, SJ
通讯作者：
Schatzberg, SJ
影响因子：
1.8
作者：
Safra, Noa;Hitchens, Peta L.;Bannasch, Danika L.
通讯作者：
Bannasch, Danika L.