Antigen persistence is required throughout the expansion phase of a CD4(+) T cell response.
Antigen persistence is required throughout the expansion phase of a CD4(+) T cell response.
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DOI：
10.1084/jem.20042521
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发表时间：
2005-05-16
期刊：
影响因子：
--
通讯作者：
Benoist C
中科院分区：
文献类型：
--
作者：
Obst R;van Santen HM;Mathis D;Benoist C
对于CD8+ T细胞，相对短的抗原脉冲似乎足以使抗原呈递细胞驱动克隆扩增和分化。目前尚不清楚CD4+ T细胞对抗原的需求是否同样短暂。为了研究在体内以定量和时间控制的方式对抗原持久性的CD4+ T细胞应答的依赖性，我们设计了一种在四环素诱导型启动子控制下在树突状细胞中表达主要组织相容性复合物II类限制性表位的小鼠系。追踪暴露于其同源抗原的不同量的CD4+ T细胞的增殖不同时间段的实验揭示，这些细胞的分裂在其整个扩增阶段取决于抗原的存在，即使在炎性刺激物的存在下。这种以前未被认识到的CD4+ T细胞应答特征与已经记录的CD8+ T细胞的增殖行为形成对比，这意味着两种T细胞亚群可能需要不同的策略来进行有效的疫苗接种。
For CD8+ T cells, a relatively short antigen pulse seems sufficient for antigen-presenting cells to drive clonal expansion and differentiation. It is unknown whether the requirement for antigen is similarly ephemeral for CD4+ T cells. To study the dependence of a CD4+ T cell response on antigen persistence in a quantitatively and temporally controlled manner in vivo, we engineered a mouse line expressing a major histocompatibility complex class II–restricted epitope in dendritic cells under the control of a tetracycline-inducible promoter. Experiments tracking the proliferation of CD4+ T cells exposed to their cognate antigen in various amounts for different time periods revealed that the division of such cells was contingent on the presence of antigen throughout their expansion phase, even in the presence of an inflammatory stimulus. This previously unrecognized feature of a CD4+ T cell response contrasts with the proliferative behavior of CD8+ T cells that has been documented, and it implies that the two T cell subsets might require different strategies for efficient vaccination.
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DOI：
10.1084/jem.194.6.769
发表时间：
2001-09-17
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Hawiger D;Inaba K;Dorsett Y;Guo M;Mahnke K;Rivera M;Ravetch JV;Steinman RM;Nussenzweig MC
通讯作者：
Nussenzweig MC
影响因子：
4.4
作者：
Faroudi, M;Zaru, R;Valitutti, S
通讯作者：
Valitutti, S
影响因子：
16.8
作者：
Friedl, P;Gunzer, M
通讯作者：
Gunzer, M
影响因子：
4.4
作者：
Bajénoff, M;Wurtz, O;Guerder, S
通讯作者：
Guerder, S
影响因子：
30.5
作者：
Kaech, SM;Ahmed, R
通讯作者：
Ahmed, R