Electroacupuncture reduces cocaine-induced seizures and mortality in mice.
Electroacupuncture reduces cocaine-induced seizures and mortality in mice.
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DOI：
10.1155/2013/134610
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Lin JG
中科院分区：
文献类型：
--
作者：
Chen YH;Ivanic B;Chuang CM;Lu DY;Lin JG
本研究的目的是研究电针(EA)对可卡因诱导的小鼠癫痫发作和死亡的保护作用。给小鼠电针(2 Hz、50 Hz、100 Hz)，或在注射可卡因前不麻醉情况下在大椎穴、百会穴行针刺。电针GV14和GV20可显著降低单次注射可卡因(75 mg/kg)诱发的癫痫发作严重程度。此外，针刺双侧GV14、GV20和电针2、50 Hz可显著降低单次致死剂量可卡因(125 mg/kg)所致的死亡率。假手术组大鼠双侧天宗穴电针频率50 ，对可卡因无保护作用。此外，电针50 HzGV14和GV20不能减少局麻药普鲁卡因所致癫痫的发生率和死亡率。在免疫组织化学研究中，电针(50赫兹)刺激GV14和GV20可降低可卡因(75 mg/kg)诱导的丘脑室旁核c-Fos表达。虽然多巴胺D3受体拮抗剂SB-277011-A(30 mg/kg，S.c)本身不影响可卡因诱导的癫痫发作的严重程度，但它阻止了电针对可卡因诱导的癫痫发作的影响。这些结果表明，电针可减轻可卡因诱导的癫痫发作和死亡率，多巴胺D3受体至少部分参与了电针的抗惊厥作用。
The aims of this study were to characterize the protective profile of electroacupuncture (EA) on cocaine-induced seizures and mortality in mice. Mice were treated with EA (2 Hz, 50 Hz, and 100 Hz), or they underwent needle insertion without anesthesia at the Dazhui (GV14) and Baihui (GV20) acupoints before cocaine administration. EA at 50 Hz applied to GV14 and GV20 significantly reduced the seizure severity induced by a single dose of cocaine (75 mg/kg; i.p.). Furthermore, needle insertion into GV14 and GV20 and EA at 2 Hz and 50 Hz at both acupoints significantly reduced the mortality rate induced by a single lethal dose of cocaine (125 mg/kg; i.p.). In the sham control group, EA at 50 Hz applied to bilateral Tianzong (SI11) acupoints had no protective effects against cocaine. In addition, EA at 50 Hz applied to GV14 and GV20 failed to reduce the incidence of seizures and mortality induced by the local anesthetic procaine. In an immunohistochemistry study, EA (50 Hz) pretreatment at GV14 and GV20 decreased cocaine (75 mg/kg; i.p.)-induced c-Fos expression in the paraventricular thalamus. While the dopamine D3 receptor antagonist, SB-277011-A (30 mg/kg; s.c), did not by itself affect cocaine-induced seizure severity, it prevented the effects of EA on cocaine-induced seizures. These results suggest that EA alleviates cocaine-induced seizures and mortality and that the dopamine D3 receptor is involved, at least in part, in the anticonvulsant effects of EA in mice.
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DOI：
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发表时间：
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期刊：
Evidence-based complementary and alternative medicine : eCAM
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