Effects of electroacupuncture of different frequencies on the release profile of endogenous opioid peptides in the central nerve system of goats.
Effects of electroacupuncture of different frequencies on the release profile of endogenous opioid peptides in the central nerve system of goats.
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不同频率电针对山羊中枢神经系统内源性阿片肽释放曲线的影响
DOI：
10.1155/2012/476457
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发表时间：
2012
期刊：
影响因子：
--
通讯作者：
Wang Q
中科院分区：
文献类型：
--
作者：
Cheng LL;Ding MX;Xiong C;Zhou MY;Qiu ZY;Wang Q
为了研究反刍动物中枢神经系统中氨基啡肽、β-内啡肽和啡肽- a的释放特征，采用0、2、40、60、80和100 Hz的电针刺激山羊30分钟。痛阈测量采用钾离子导入法。用SABC免疫组化法测定多肽水平。结果表明，60 Hz使疼痛阈值增加91%；其增重率高于其他各频率（P < 0.01）。2 Hz和100 Hz使伏隔核、间隔区、尾状核、杏仁核、下丘脑室旁核、导水管周围灰核、中缝背核和蓝斑核的脑啡肽免疫活性升高（P < 0.05）。两种频率诱发伏隔核、隔区、视上核、下丘脑腹内侧核、导水管周围灰核、中缝背核、蓝斑核、孤立核和杏仁核释放β-内啡肽（P < 0.05）。60 Hz时，上述核区及束核、黑质、臂旁核、中缝大核的脑啡肽、β-内啡肽免疫活性均升高（P < 0.05）。高频刺激可增加脊髓背角及大部分镇痛相关核的肌啡肽- a释放（P < 0.05）。提示60hz可诱导三种多肽在中枢神经系统广泛的镇痛相关核和区域同时释放，这可能是导致最佳镇痛效果和物种变异的原因。
To investigate the release profile of met-enkephalin, β-endorphin, and dynorphin-A in ruminants' CNS, goats were stimulated by electroacupuncture of 0, 2, 40, 60, 80, or 100 Hz for 30 min. The pain threshold was measured using potassium iontophoresis. The peptide levels were determined with SABC immunohistochemisty. The results showed that 60 Hz increased pain threshold by 91%; its increasing rate was higher (P < 0.01) than any other frequency did. 2 Hz and 100 Hz increased met-enkephalin immunoactivities (P < 0.05) in nucleus accumbens, septal area, caudate nucleus, amygdala, paraventricular nucleus of hypothalamus, periaqueductal gray, dorsal raphe nucleus, and locus ceruleus. The two frequencies elicited β-endorphin release (P < 0.05) in nucleus accumbens, septal area, supraoptic nucleus, ventromedial nucleus of hypothalamus, periaqueductal gray, dorsal raphe nucleus, locus ceruleus, solitary nucleus and amygdala. 60 Hz increased (P < 0.05) met-enkephalin or β-endorphin immunoactivities in the nuclei and areas mentioned above, and habenular nucleus, substantia nigra, parabrachial nucleus, and nucleus raphe magnus. High frequencies increased dynorphin-A release (P < 0.05) in spinal cord dorsal horn and most analgesia-related nuclei. It suggested that 60 Hz induced the simultaneous release of the three peptides in extensive analgesia-related nuclei and areas of the CNS, which may be contributive to optimal analgesic effects and species variation.
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影响因子：
2.9
作者：
GRAMSCH, C;HOLLT, V;HERZ, A
通讯作者：
HERZ, A
影响因子：
2.5
作者：
Han, Z;Jiang, YH;Han, JS
通讯作者：
Han, JS
影响因子：
3.5
作者：
Cho, ZH;Hwang, SC;Min, KT
通讯作者：
Min, KT
影响因子：
2.9
作者：
BAUMEISTER, AA;ANTICICH, TG;GUILLORY, EC
通讯作者：
GUILLORY, EC
影响因子：
2.4
作者：
Grant, C;Upton, RN
通讯作者：
Upton, RN