Healing of Preterm Ruptured Fetal Membranes.
Healing of Preterm Ruptured Fetal Membranes.
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DOI：
10.1038/s41598-017-13296-1
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发表时间：
2017-10-13
影响因子：
4.6
通讯作者：
Word RA
中科院分区：
文献类型：
--
作者：
Mogami H;Hari Kishore A;Akgul Y;Word RA
早产胎膜早破（premature premature rupture of membrane，pPROM）与30-40%的早产有关。由于羊水中促炎细胞因子水平升高，感染被认为是胎膜早破的主要原因。然而，只有30%的羊水培养微生物阳性。有趣的是，在一些妊娠并发早产胎膜早破（pPROM），膜自发愈合，妊娠持续到足月。在这里，我们研究了羊膜愈合的机制。使用临床前小鼠模型，我们发现胎膜小破裂在72小时内闭合，而大破裂的愈合率仅为40%。小破裂引起细胞因子的瞬时上调，而大破裂引起持续上调的促炎细胞因子在胎膜。羊水中的胎儿巨噬细胞被募集到巨噬细胞粘附分子高度表达的损伤羊膜中。募集的巨噬细胞释放有限且定位良好的量的IL-1β和TNF，其促进上皮-间充质转化（EMT）和上皮细胞迁移。Arg 1+巨噬细胞在24 h内占优势。然而，羊膜间充质室的迁移和愈合仍然受到影响。这些发现为羊膜独特的愈合机制提供了新的见解。
Preterm premature rupture of membrane (pPROM) is associated with 30–40% of preterm births. Infection is considered a leading cause of pPROM due to increased levels of proinflammatory cytokines in amniotic fluid. Only 30%, however, are positive for microbial organisms by amniotic fluid culture. Interestingly, in some pregnancies complicated by preterm premature rupture of membranes (pPROM), membranes heal spontaneously and pregnancy continues until term. Here, we investigated mechanisms of amnion healing. Using a preclinical mouse model, we found that small ruptures of the fetal membrane closed within 72 h whereas healing of large ruptures was only 40%. Small rupture induced transient upregulation of cytokines whereas large ruptures elicited sustained upregulation of proinflammatory cytokines in the fetal membranes. Fetal macrophages from amniotic fluid were recruited to the wounded amnion where macrophage adhesion molecules were highly expressed. Recruited macrophages released limited and well-localized amounts of IL-1β and TNF which facilitated epithelial-mesenchymal transition (EMT) and epithelial cell migration. Arg1 + macrophages dominated within 24 h. Migration and healing of the amnion mesenchymal compartment, however, remained compromised. These findings provide novel insights regarding unique healing mechanisms of amnion.
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影响因子：
17.1
作者：
Eming SA;Martin P;Tomic-Canic M
通讯作者：
Tomic-Canic M
影响因子：
6.5
作者：
Campbell, Laura;Saville, Charis R.;Murray, Peter J.;Cruickshank, Sheena M.;Hardman, Matthew J.
通讯作者：
Hardman, Matthew J.
DOI：
10.1038/nri3073
发表时间：
2011-10-14
期刊：
Nature reviews. Immunology
影响因子：
--
作者：
通讯作者：
--
影响因子：
9.8
作者：
Borgida, AF;Mills, AA;Egan, JFX
通讯作者：
Egan, JFX
DOI：
10.1016/j.jsgi.2005.08.008
发表时间：
2005-12-01
期刊：
JOURNAL OF THE SOCIETY FOR GYNECOLOGIC INVESTIGATION
影响因子：
--
作者：
Cheung, CY;Brace, RA
通讯作者：
Brace, RA