Exploring the host parasitism of the migratory plant-parasitic nematode Ditylenchus destuctor by expressed sequence tags analysis.
Exploring the host parasitism of the migratory plant-parasitic nematode Ditylenchus destuctor by expressed sequence tags analysis.
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通过表达序列标签分析探索迁徙植物寄生线虫茎线虫的寄主寄生。
DOI：
10.1371/journal.pone.0069579
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Peng DL
中科院分区：
文献类型：
--
作者：
Peng H;Gao BL;Kong LA;Yu Q;Huang WK;He XF;Long HB;Peng DL
马铃薯腐烂线虫是危害世界各地重要农业作物的一种极具破坏性的线虫，但对其寄生植物的分子生物学特性研究较少。在过去的二十年里，人们已经发现并广泛研究了几种寄生在植物体内的线虫的影响因子，如大豆异型线虫、罗氏大斑线虫和南方根结线虫。作为一种迁移性植物寄生线虫，其取食行为、生活史和寄主反应各不相同。比较不同类型植物寄生线虫的转录组和寄生体组是更全面了解植物线虫寄生机制的途径。我们采用测序的方法对来源于D-destructor混合阶段文库的表达序列标签(EST)进行了测序。这是对D.destructor Est的首次研究。共有9800个EST被分成5008个簇，其中包括3606个单态和1402个多成员重叠群，代表了一个解构杆菌基因的目录。在生物信息学的工作流程中，我们发现1391个簇在现有的基因数据库中没有匹配；31个簇仅与金线虫(D.africanus)鉴定的基因有相似性；1991个簇被用基因本体论(GO)注释；1550个簇被分配了酶委员会(EC)编号；1211个簇被映射到181个KEGG生化途径。22个EST与已报道的线虫效应器相似。有趣的是，本研究中鉴定的大多数效应物都参与了宿主细胞壁的降解或修饰，如1，4-β-葡聚糖、1，3-β-葡聚糖、果胶裂解酶、几丁质酶和膨胀素，或者参与了宿主防御抑制，如钙网蛋白、膜联蛋白和蛇毒变应原样蛋白。这一结果表明，迁徙的植物寄生线虫D.destructor分泌了与定居植物线虫相似的效应。最后，我们进一步鉴定了这两种D破坏因子扩张素蛋白。
The potato rot nematode, Ditylenchus destructor, is a very destructive nematode pest on many agriculturally important crops worldwide, but the molecular characterization of its parasitism of plant has been limited. The effectors involved in nematode parasitism of plant for several sedentary endo-parasitic nematodes such as Heterodera glycines, Globodera rostochiensis and Meloidogyne incognita have been identified and extensively studied over the past two decades. Ditylenchus destructor, as a migratory plant parasitic nematode, has different feeding behavior, life cycle and host response. Comparing the transcriptome and parasitome among different types of plant-parasitic nematodes is the way to understand more fully the parasitic mechanism of plant nematodes. We undertook the approach of sequencing expressed sequence tags (ESTs) derived from a mixed stage cDNA library of D. destructor. This is the first study of D. destructor ESTs. A total of 9800 ESTs were grouped into 5008 clusters including 3606 singletons and 1402 multi-member contigs, representing a catalog of D. destructor genes. Implementing a bioinformatics' workflow, we found 1391 clusters have no match in the available gene database; 31 clusters only have similarities to genes identified from D. africanus, the most closely related species to D. destructor; 1991 clusters were annotated using Gene Ontology (GO); 1550 clusters were assigned enzyme commission (EC) numbers; and 1211 clusters were mapped to 181 KEGG biochemical pathways. 22 ESTs had similarities to reported nematode effectors. Interestedly, most of the effectors identified in this study are involved in host cell wall degradation or modification, such as 1,4-beta-glucanse, 1,3-beta-glucanse, pectate lyase, chitinases and expansin, or host defense suppression such as calreticulin, annexin and venom allergen-like protein. This result implies that the migratory plant-parasitic nematode D. destructor secrets similar effectors to those of sedentary plant nematodes. Finally we further characterized the two D. destructor expansin proteins.
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