Construction of a high-density microsatellite genetic linkage map and mapping of sexual and growth-related traits in half-smooth tongue sole (Cynoglossus semilaevis).
Construction of a high-density microsatellite genetic linkage map and mapping of sexual and growth-related traits in half-smooth tongue sole (Cynoglossus semilaevis).
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DOI：
10.1371/journal.pone.0052097
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Chen S
中科院分区：
文献类型：
--
作者：
Song W;Li Y;Zhao Y;Liu Y;Niu Y;Pang R;Miao G;Liao X;Shao C;Gao F;Chen S
半滑舌鳎高密度遗传连锁图谱包含1007个微卫星标记、2个SCAR标记和94个F1家族。雌性图谱由21个连锁群828个标记组成，总长度为1447.3 cM，标记间平均间隔为1.83 cM。雄性图谱由21个连锁群中的794个标记组成，跨度为1497.5 cM，平均间隔为1.96 cM。女性和男性地图分别有 812 个和 785 个独特位置。半滑舌鳎的基因组长度估计雌性为 1527.7 cM，雄性为 1582.1 cM。根据对图谱长度的估计，女性和男性图谱分别覆盖了基因组的 94.74% 和 94.65%。共有图谱由21个连锁群的1007个微卫星标记和2个SCAR标记组成，共覆盖1624 cM，平均间隔1.67 cM。此外，还鉴定了 159 个与性别相关的 SSR 标记。在选自不同家庭的大量个体中，证实了五个与性别相关的微卫星标记与性别的关联。这些性别连锁标记以零重组的方式绘制在雌性图谱 LG1f 上。两个与体重相关的QTL，命名为We-1和We-2，分别占表型变异的26.39%和10.60%。 LG4f 中定位了两个体宽 QTL，指定为 Wi-1 和 Wi-2，分别占表型变异的 14.33% 和 12.83%。通过CIM在LG1f、LG14f和LG1m中定位到7个性别相关位点，占性状变异的12.5%~25.2%。结果应该被证明对于利用分子 MAS 改善生长性状非常有用。
High-density genetic linkage maps of half-smooth tongue sole were developed with 1007 microsatellite markers, two SCAR markers and an F1 family containing 94. The female map was composed of 828 markers in 21 linkage groups, covering a total of 1447.3 cM, with an average interval 1.83 cM between markers. The male map consisted of 794 markers in 21 linkage groups, spanning 1497.5 cM, with an average interval of 1.96 cM. The female and male maps had 812 and 785 unique positions, respectively. The genome length of half-smooth tongue sole was estimated to be 1527.7 cM for the females and 1582.1 cM for the males. Based on estimations of the map lengths, the female and male maps covered 94.74 and 94.65% of the genome, respectively. The consensus map was composed of 1007 microsatellite markers and two SCAR markers in 21 linkage groups, covering a total of 1624 cM with an average interval of 1.67 cM. Furthermore, 159 sex-linked SSR markers were identified. Five sex-linked microsatellite markers were confirmed in their association with sex in a large number of individuals selected from different families. These sex-linked markers were mapped on the female map LG1f with zero recombination. Two QTLs that were identified for body weight, designated as We-1 and We-2, accounted for 26.39% and 10.60% of the phenotypic variation. Two QTLs for body width, designated Wi-1 and Wi-2, were mapped in LG4f and accounted for 14.33% and 12.83% of the phenotypic variation, respectively. Seven sex-related loci were mapped in LG1f, LG14f and LG1m by CIM, accounting for 12.5–25.2% of the trait variation. The results should prove to be very useful for improving growth traits using molecular MAS.
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影响因子：
4.4
作者：
Guyomard, Rene;Mauger, Stephane;Tabet-Canale, Kamila;Martineau, Sylvain;Genet, Carine;Krieg, Francine;Quillet, Edwige
通讯作者：
Quillet, Edwige
影响因子：
3.3
作者：
Kai, W;Kikuchi, K;Suzuki, Y
通讯作者：
Suzuki, Y
影响因子：
3.3
作者：
Hubert, S;Hedgecock, D
通讯作者：
Hedgecock, D
影响因子：
3.1
作者：
Cervantes-Flores, Jim C.;Yencho, G. Craig;Sosinski, Bryon R.
通讯作者：
Sosinski, Bryon R.
影响因子：
3.3
作者：
Lee, BY;Lee, WJ;Kocher, TD
通讯作者：
Kocher, TD