Telocytes in human epicardium.
Telocytes in human epicardium.
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DOI：
10.1111/j.1582-4934.2010.01129.x
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发表时间：
2010-08
影响因子：
5.3
通讯作者：
Kostin S
中科院分区：
文献类型：
--
作者：
Popescu LM;Manole CG;Gherghiceanu M;Ardelean A;Nicolescu MI;Hinescu ME;Kostin S
先前通过免疫组织化学 (IHC) 或免疫荧光记录了心外膜端细胞的存在。我们最近还证明，远程细胞存在于小鼠心外膜中的心脏干细胞生态位内，并且它们可能充当心肌祖细胞的护士细胞。这项研究的基本原理是证明人类心外膜（下）外膜中也确实存在远程细胞。来自 10 名成人和 15 名胎儿的人体尸检心脏用于 c-kit/CD117、CD34、波形蛋白、S-100、τ、神经激肽 1 的常规 IHC，以及使用激光共聚焦显微镜。通过数字透射电子显微镜 (TEM) 对 10 名成人的手术活检获得的组织样本进行了研究。 c-kit/CD34 和 c-kit/波形蛋白的双重免疫标记表明，在人类中，心外膜特细胞与心肌特细胞具有相似的免疫表型特征。 TEM 显示人心外膜中存在远程细胞。心外膜远程细胞，像任何远程细胞一样，由端足定义，它们的细胞延长非常长（几十微米），非常薄（0.1-0.2微米，低于光学显微镜的分辨率）并且具有念珠状结构。相互连接的心外膜远程细胞创建了一个 3D 细胞网络，与心肌远程细胞的 3D 网络相连。 TEM 记录了远程细胞在细胞间隙中释放脱落的微泡或胞吐多泡体。人类心外膜特细胞与位于人类工作心肌细胞中的特细胞具有相似的表型（TEM 和 IHC）。特细胞在心脏更新/修复/再生过程中的作用，以及它们的“功能抑制”或其数量变化引起的病理方面仍有待确定。我们认为特细胞是心脏病自体细胞疗法未来发展的真正候选者。
The existence of the epicardial telocytes was previously documented by immunohistochemistry (IHC) or immunofluorescence. We have also demonstrated recently that telocytes are present in mice epicardium, within the cardiac stem-cell niches, and, possibly, they are acting as nurse cells for the cardiomyocyte progenitors. The rationale of this study was to show that telocytes do exist in human (sub)epicardium, too. Human autopsy hearts from 10 adults and 15 foetuses were used for conventional IHC for c-kit/CD117, CD34, vimentin, S-100, τ, Neurokinin 1, as well as using laser confocal microscopy. Tissue samples obtained by surgical biopsies from 10 adults were studied by digital transmission electron microscopy (TEM). Double immunolabelling for c-kit/CD34 and, for c-kit/vimentin suggests that in human beings, epicardial telocytes share similar immunophenotype features with myocardial telocytes. The presence of the telocytes in human epicardium is shown by TEM. Epicardial telocytes, like any of the telocytes are defined by telopodes, their cell prolongations, which are very long (several tens of μm), very thin (0.1–0.2 μm, below the resolving power of light microscopy) and with moniliform configuration. The interconnected epicardial telocytes create a 3D cellular network, connected with the 3D network of myocardial telocytes. TEM documented that telocytes release shed microvesicles or exocytotic multivesicular bodies in the intercellular space. The human epicardial telocytes have similar phenotype (TEM and IHC) with telocytes located among human working cardiomyocyte. It remains to be established the role(s) of telocytes in cardiac renewing/repair/regeneration processes, and also the pathological aspects induced by their ‘functional inhibition’, or by their variation in number. We consider telocytes as a real candidate for future developments of autologous cell-based therapy in heart diseases.
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影响因子：
5.3
作者：
Kostin S
通讯作者：
Kostin S
DOI：
10.1111/j.1582-4934.2006.tb00306.x
发表时间：
2006-01
影响因子：
5.3
作者：
Hinescu ME;Gherghiceanu M;Mandache E;Ciontea SM;Popescu LM
通讯作者：
Popescu LM
影响因子：
3.2
作者：
Hinescu, Mihail E.;Ardeleanu, Carmen;Popescu, Laurentziu M.
通讯作者：
Popescu, Laurentziu M.
影响因子：
5
作者：
Limana, Federica;Bertolami, Chiara;Capogrossi, Maurizio C.
通讯作者：
Capogrossi, Maurizio C.
产前和产后生活期间端细胞和心肌细胞之间的关系。
影响因子：
5.3
作者：
Faussone-Pellegrini MS;Bani D
通讯作者：
Bani D