Availability of Micro-Tom mutant library combined with TILLING in molecular breeding of tomato fruit shelf-life.
Availability of Micro-Tom mutant library combined with TILLING in molecular breeding of tomato fruit shelf-life.
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DOI：
10.1270/jsbbs.62.202
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发表时间：
2012-06
期刊：
影响因子：
2.4
通讯作者：
Ezura H
中科院分区：
文献类型：
--
作者：
Okabe Y;Asamizu E;Ariizumi T;Shirasawa K;Tabata S;Ezura H
本研究利用TILLING技术从Micro-Tom突变体库中分离到番茄乙烯受体基因（Solanum lycopersicum ETHYLENE RESPONSE 1，SlETR 1）的新突变等位基因Sletr 1 -1和Sletr 1 -2。这些等位基因表现出不同程度的果实成熟受损表型，表明这些等位基因可能是提高番茄果实货架期的有价值的育种材料。为了在番茄育种中实际使用Sletr 1等位基因，需要通过转化携带每个等位基因的基因进行遗传互补分析。在这项研究中，我们产生并表征过表达Sletr 1 -1和Sletr 1 -2的转基因株系。所有转基因株系均表现出乙烯不敏感表型和成熟抑制，表明Sletr 1 -1和Sletr 1 -2与乙烯不敏感表型相关。结果表明，转基因株系与转基因株系的乙烯敏感性存在差异，但果实成熟表型无明显差异。这些结果表明，即使使用较弱的等位基因（Sletr 1 -2），也难以通过转基因方法微调成熟程度。本研究表明，Micro-Tom突变体库与TILLING技术相结合，可作为番茄育种中重要农艺性状遗传变异的有效工具。
Novel mutant alleles of an ethylene receptor Solanum lycopersicum ETHYLENE RESPONSE1 (SlETR1) gene, Sletr1-1 and Sletr1-2, were isolated from the Micro-Tom mutant library by TILLING in our previous study. They displayed different levels of impaired fruit ripening phenotype, suggesting that these alleles could be a valuable breeding material for improving shelf life of tomato fruit. To conduct practical use of the Sletr1 alleles in tomato breeding, genetic complementation analysis by transformation of genes carrying each allele is required. In this study, we generated and characterized transgenic lines over-expressing Sletr1-1 and Sletr1-2. All transgenic lines displayed ethylene insensitive phenotype and ripening inhibition, indicating that Sletr1-1 and Sletr1-2 associate with the ethylene insensitive phenotype. The level of ethylene sensitivity in the seedling was different between Sletr1-1 and Sletr1-2 transgenic lines, whereas no apparent difference was observed in fruit ripening phenotype. These results suggested that it is difficult to fine-tune the extent of ripening by transgenic approach even if the weaker allele (Sletr1-2) was used. Our present and previous studies indicate that the Micro-Tom mutant library combined with TILLING could be an efficient tool for exploring genetic variations of important agronomic traits in tomato breeding.
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影响因子：
--
作者：
Mueller, LA;Tanksley, SD;Giovannoni, JJ;van Eck, J;Stack, S;Choi, D;Kim, BD;Chen, MS;Cheng, ZK;Li, CY;Ling, HQ;Xue, YB;Seymour, G;Bishop, G;Bryan, G;Sharma, R;Khurana, J;Tyagi, A;Chattopadhyay, D;Singh, NK;Stiekema, W;Lindhout, P;Jesse, T;Lankhorst, RK;Bouzayen, M;Shibata, D;Tabata, S;Granell, A;Botella, MA;Giullano, G;Frusciante, L;Causse, M;Zamir, D
通讯作者：
Zamir, D
LeERF1正向调节番茄黄化幼苗、植物发育以及果实成熟和软化的乙烯三重反应
影响因子：
6.2
作者：
Li, Yingcong;Zhu, Benzhong;Luo, Yunbo
通讯作者：
Luo, Yunbo
影响因子：
7.4
作者：
Gallie, Daniel R.
通讯作者：
Gallie, Daniel R.
影响因子：
7.4
作者：
Mueller, LA;Solow, TH;Tanksley, SD
通讯作者：
Tanksley, SD
影响因子：
7.2
作者：
Meissner, R;Jacobson, Y;Levy, A
通讯作者：
Levy, A