Oxytocin and vasopressin involved in restraint water-immersion stress mediated by oxytocin receptor and vasopressin 1b receptor in rat brain.
Oxytocin and vasopressin involved in restraint water-immersion stress mediated by oxytocin receptor and vasopressin 1b receptor in rat brain.
复制标题
催产素和加压素参与大鼠脑内催产素受体和加压素 1b 受体介导的束缚水浸应激
DOI：
10.1371/journal.pone.0023362
复制
发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Ai HB
中科院分区：
文献类型：
--
作者：
Zhao DQ;Ai HB
目的加压素(AVP)和催产素(OT)被认为与胃功能和应激反应的调节有关。本研究旨在探讨加压素能神经元和催产素能神经元在束缚水浸应激中的作用。方法雄性Wistar大鼠10只，随机分为对照组和RWI1h组。Fos和催产素(OT)或加压素(AVP)或OT受体或AVP 1b受体(V1bR)双重免疫组织化学染色。结果(1)RWIS大鼠下丘脑室旁核(PVN)、视上核(SON)、孤束核(NTS)和迷走神经运动核(DMV)Fos免疫反应阳性(Fos-IR)神经元明显增多，OT免疫反应(OT-IR)神经元主要见于PVN内侧大细胞部和SON背侧，AVP免疫反应(AVP-IR)神经元主要分布于PVN大细胞部和SON腹侧。在RWIS大鼠，31%的OT-IR神经元和40%的AVP-IR神经元在PVN内可见Fos-IR神经元，而在SON中Fos-IR神经元的比例分别为28%和53%：(3)V1bR-IR和OTR-IR神经元占据了NTS和DMV的全部。在RWIS大鼠，超过10%的OTR-IR和V1bR-IR神经元在DMV被激活，而在NTS被激活的比例较低。结论RWIs可同时激活PVN和SON内的催产素能神经元和加压素能神经元，并可能投射到NTS或DMV，通过OTR和V1bR介导神经元的活动。
Aims Vasopressin (AVP) and oxytocin (OT) are considered to be related to gastric functions and the regulation of stress response. The present study was to study the role of vasopressinergic and oxytocinergic neurons during the restraint water-immersion stress. Methods Ten male Wistar rats were divided into two groups, control and RWIS for 1h. The brain sections were treated with a dual immunohistochemistry of Fos and oxytocin (OT) or vasopressin (AVP) or OT receptor or AVP 1b receptor (V1bR). Results (1) Fos-immunoreactive (Fos-IR) neurons dramatically increased in the hypothalamic paraventricular nucleus (PVN), the supraoptic nucleus (SON), the neucleus of solitary tract (NTS) and motor nucleus of the vagus (DMV) in the RWIS rats; (2) OT-immunoreactive (OT-IR) neurons were mainly observed in the medial magnocellular part of the PVN and the dorsal portion of the SON, while AVP-immunoreactive (AVP-IR) neurons mainly distributed in the magnocellular part of the PVN and the ventral portion of the SON. In the RWIS rats, Fos-IR neurons were indentified in 31% of OT-IR neurons and 40% of AVP-IR neurons in the PVN, while in the SON it represented 28%, 53% respectively; (3) V1bR-IR and OTR-IR neurons occupied all portions of the NTS and DMV. In the RWIS rats, more than 10% of OTR-IR and V1bR-IR neurons were activated in the DMV, while lower ratio in the NTS. Conclusion RWIS activates both oxytocinergic and vasopressinergic neurons in the PVN and SON, which may project to the NTS or DMV mediating the activity of the neurons by OTR and V1bR.
登录
查看更多内容
影响因子：
4.2
作者：
Pirnik, Z;Mravec, B;Kiss, A
通讯作者：
Kiss, A
影响因子：
2.9
作者：
BONAZ, B;TACHE, Y
通讯作者：
TACHE, Y
影响因子：
2.9
作者：
du Plessis, Irne;Mitchell, Duncan;Laburn, Helen P.
通讯作者：
Laburn, Helen P.
影响因子：
2.9
作者：
GIOVANNELLI, L;SHIROMANI, PJ;BLOOM, FE
通讯作者：
BLOOM, FE
影响因子：
2.9
作者：
Harrison, TA
通讯作者：
Harrison, TA