Flavone ingredients can synergistically inhibit NDV infecting cell and improve ND vaccine's protective rate.
Flavone ingredients can synergistically inhibit NDV infecting cell and improve ND vaccine's protective rate.
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DOI：
10.1016/j.ijbiomac.2012.04.028
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发表时间：
2012-10
影响因子：
8.2
通讯作者：
Yuanliang H
中科院分区：
文献类型：
--
作者：
Yuqing Z;Zhan'ao S;Jiaguo L;Deyun W;Baokang Z;Fang Y;Yunpeng F;Dan L;Xu L;Cui L;Yuanliang H
黄酮类药物配伍合理，具有协同抗病毒作用。EF-SF-WDFF-BSRF、EF-SF-WDFF-BSRF和EF-WDFF-BSRF能显著抑制NDV对CEF的细胞感染。BSRF-WDFF-SF-WDFF-BSRF可提高ND疫苗的保护效果。BSRF有望开发成为一种新型的抗病毒药物。为筛选抗新城疫病毒（NDV）的黄酮类药物处方，将前期筛选的淫羊藿黄酮（EF）、黄芩黄酮（BSRF）、野菊花黄酮（WDFF）、三七黄酮（SF）4种黄酮类成分及其处方分别以3种加药方式加入鸡胚成纤维细胞单层中。计算细胞A570值、最高病毒抑制率和基于病毒抑制率的评分，以比较它们的抗病毒活性。在免疫保护试验中，比较了经体外试验筛选出的EF-BSRF、EF-SF-WDFF-BSRF和EF-WDFF-BSRF 3种制剂对新城疫疫苗免疫后再攻毒的效果。定期采集血样进行血清抗体滴度测定。临床检查鸡的致病和死亡情况。结果表明，9个方剂，尤其是上述3个方剂在3种加药方式下几乎所有浓度的A570值均显著高于病毒对照组。上述三个方的病毒抑制率均位于最高的前五位，得分排名均位于最高的前三位。3个剂量组的抗体效价和保护率均高于VC组，其中EF-SF-WDFF-BSRF组差异有统计学意义。结果表明，黄酮类药物配伍后具有协同抗病毒作用，ES-SF-WDFF-BSRF复方可抑制NDV的细胞感染性，提高ND疫苗的保护效果，有望开发成为新型抗病毒药物。
► Flavone prescriptions composed with suitable compatibility could possess synergistical action of antiviral effect. ► EF-BSRF, EF-SF-WDFF-BSRF and EF-WDFF-BSRF could significantly inhibit the cellular infectivity of NDV in CEF. ► EF-SF-WDFF-BSRF could improve the protective effect of ND vaccine. ► EF-SF-WDFF-BSRF would be expected to exploit into a new-type antiviral drug. In order to screen better flavone prescriptions of anti-Newcastle disease virus (NDV), four flavone ingredients of epimedium flavones (EF), baikal skullcap root flavones (BSRF), wild dendranthema flower flavones (WDFF), and sanchi flavones (SF) screened in previous experiments and their prescriptions were added into chicken embryo fibroblast monolayer with three drug-adding modes respectively. The cellular A570 values, the highest virus inhibitory rates and the score based on virus inhibitory rate were calculated to compare their antiviral activity. In immune protective test, the effects of three preparations (EF-BSRF, EF-SF-WDFF-BSRF and EF-WDFF-BSRF screened by the results in vitro experiment) on NDV infection were compared in chickens vaccinated with ND vaccine then challenged with NDV. Blood was regularly sampled for serum antibody titer determination. The pathogenic and dead statuses of chickens were clinically examined. The results indicated that the A570 values of the nine prescriptions, especially the foresaid three prescriptions at almost all concentrations in three drug-adding modes were significantly higher than that of the virus control group. The foresaid three prescriptions presented at the top five of the highest virus inhibitory rate, and located at the highest three of the score rank. The antibody titers and protective rates of the three prescriptions groups were higher than that of VC group, especially EF-SF-WDFF-BSRF group showed significant difference. These results indicated that flavone prescriptions composed with suitable compatibility could possess synergistical action of antiviral effect, ES-SF-WDFF-BSRF prescription could inhibit the cellular infectivity of NDV, improve the protective effect of ND vaccine and would be expected to exploit into a new-type antiviral drug.
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