Isolation, characterization, and identification of proteins interfering with enzyme-linked immunosorbent assay of antibiotics in fish matrix
Isolation, characterization, and identification of proteins interfering with enzyme-linked immunosorbent assay of antibiotics in fish matrix
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干扰鱼基质中抗生素酶联免疫吸附测定的蛋白质的分离、表征和鉴定
DOI：
10.1007/s10068-016-0200-x
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发表时间：
2016-10
影响因子：
2.9
通讯作者：
Sui Jianxin
中科院分区：
文献类型：
--
作者：
Zheng Hongwei;Cui Mengqi;Du Shuyuan;Sui Jianxin
某些蛋白质与免疫球蛋白之间的相互作用已被证实是水产品免疫分析中基质干扰的重要来源，但这些蛋白质的详细生化特性尚未得到说明。从比目鱼中分离、鉴定了两个干扰诱导蛋白（42和36 kD）。通过蛋白质印迹法和酶联免疫吸附试验证实了它们对诺氟沙星免疫测定的影响。并对两种蛋白的pI值和pH稳定性进行了研究。经LC-MS/MS鉴定，这两种蛋白均为果糖二磷酸醛缩酶，并通过42和36 kD菌株的醛缩酶活性得到部分验证。考虑到这些蛋白质（作为肌肉的多功能醛缩酶）在食品中的普遍存在，这些结果将有助于进一步了解各种免疫测定中的基质效应，以及开发有效的技术来提高免疫测定的效率。
The interaction between some proteins and immune globulin has been confirmed as an important source of matrix interference with the immunoassay of fishery products, but detailed biochemical properties of these proteins have not been indicated. Two interference-inducing proteins (42 and 36 kD) in flounder were isolated, characterized, and identified. Their influences on the immunoassay of norfloxacin were confirmed by western blotting and enzyme-linked immunosorbent assay. The pI value and pH stability of the two proteins were also investigated. Using LC-MS/MS, the two proteins were identified as fructose-bisphosphate aldolase, and such results were partly verified by the aldolase activity of the 42 and 36 kD isolates. Considering the prevalence of these proteins (as multi-functional aldolase of muscles) in foods, these results would help to further understand the matrix effects in various immunoassays as well as the development of effective techniques to improve the efficiency of immunoassays.
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影响因子：
--
作者：
Mengqi Cui;Hong Lin;Xiudan Wang;Limin Cao;Jianxin Sui
通讯作者：
Mengqi Cui;Hong Lin;Xiudan Wang;Limin Cao;Jianxin Sui
影响因子：
6.1
作者：
S. Richman;S. Karthikeyan;D. A. Bennett;A. C. Chung;S. M. Lee
通讯作者：
S. Richman;S. Karthikeyan;D. A. Bennett;A. C. Chung;S. M. Lee
影响因子：
4.2
作者：
Johansson, MA;Hellenäs, KE
通讯作者：
Hellenäs, KE
影响因子：
3
作者：
Wang, Xiudan;Lin, Hong;Sui, Jianxin
通讯作者：
Sui, Jianxin
影响因子：
6.2
作者：
Cacciatore, G;Petz, M;Bergwerff, AA
通讯作者：
Bergwerff, AA