Molecular characterization of a novel monopartite begomovirus isolated from Pouzolzia zeylanica in China

Molecular characterization of a novel monopartite begomovirus isolated from Pouzolzia zeylanica in China
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从中国的 Pouzolzia zeylanica 中分离出的新型单部分 Begomovirus 的分子特征

DOI:
10.1007/s00705-013-1632-8
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发表时间:
2013-03
影响因子:
2.7
通讯作者:
J.H. Cai
J.H. Cai
中科院分区:
医学4区
文献类型:
--
作者:
Z.G. Du;Z.F. He;X. M. She;J.H. Cai

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单部分 begomovirus 分离株 TY01 的完整基因组序列是从中国广西省百色表现出金色花叶症状的患病 Pouzolzia zeylanica 植物中获得的。它由 2723 个核苷酸 (nt) 组成,编码病毒颗粒正义 DNA 中的两个 ORF(CP 和 AV2)和互补正义 DNA 中的五个 ORF(AC1-AC5)。与其他Begomovirus的DNA-A序列相比,它与来自泰国的Ageratum黄脉病毒(AYVV)分离株AFSP6D具有最高的(78.5%)核苷酸序列一致性,低于双子病毒科Begomovirus属物种划分阈值的完整基因组89%的一致性。系统发育分析表明,TY01 与其他 28 个 Begomovirus 分离株分属一个独立的分支。这些结果表明分离株 TY01 是一种新的 Begomovirus 物种的成员,建议将其命名为“Pouzolzia 金色花叶病毒”(PGMV)。
The complete genome sequence of a monopartite begomovirus isolate TY01 was obtained from diseased Pouzolzia zeylanica plants exhibiting golden mosaic symptoms in Baise, Guangxi Province, China. It consisted of 2723 nucleotides (nt) and encoded two ORFs (CP and AV2) in the virion-sense DNA and five ORFs (AC1-AC5) in the complementary-sense DNA. Compared with the DNA-A sequences of other begomoviruses, it has the highest (78.5 %) nucleotide sequence identity with ageratum yellow vein virus (AYVV) isolate AFSP6D from Thailand, which is less than the 89 % identity in the complete genome that has been defined as the threshold value for demarcation of species in the genus Begomovirus, family Geminiviridae. Phylogenetic analysis showed that TY01 was grouped in a separate clade from the other 28 begomovirus isolates. These results indicate that isolate TY01 is a member of a novel Begomovirus species, for which the name "Pouzolzia golden mosaic virus" (PGMV) is proposed.
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