Candida albicans forms biofilms on the vaginal mucosa.
Candida albicans forms biofilms on the vaginal mucosa.
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白色念珠菌在阴道粘膜上形成生物膜。
DOI：
10.1099/mic.0.039354-0
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发表时间：
2010-12
期刊：
影响因子：
--
通讯作者：
Noverr MC
中科院分区：
文献类型：
--
作者：
Harriott MM;Lilly EA;Rodriguez TE;Fidel PL;Noverr MC
目前对外阴阴道念珠菌病的抗性和易感性的了解挑战了宿主防御真菌感染的现有范式。虽然非生物生物膜的形成在全身性念珠菌感染中具有明确的作用，但尚不清楚白色念珠菌是否在阴道粘膜上形成生物膜，以及生物膜在疾病中的可能作用。用扫描电子显微镜和共聚焦显微镜观察小鼠阴道炎在体内和离体模型生物膜的形成。白色念珠菌菌株包括3153A（实验室菌株）、DAY185（亲本对照菌株）和体外形态发生和/或生物膜形成缺陷的突变体（efg1/efg1和bcr1/bcr1）。3153A和DAY815在体内和离体阴道粘膜上形成生物膜，高真菌负荷和显微镜分析表明典型的生物膜结构和细胞外基质（ECM）的存在与真菌的存在共定位。相比之下，尽管定植水平相当，但efg1/efg1和bcr1/bcr1突变株在两种模型中都表现出较弱或没有生物膜形成/ECM产生。这些数据首次表明白色念珠菌在体内阴道上皮上形成生物膜，并且在体内生物生物膜的形成需要生物膜形成调控因子（BCR1）和形态发生调控因子（EFG1）。
Current understanding of resistance and susceptibility to vulvovaginal candidiasis challenges existing paradigms of host defence against fungal infection. While abiotic biofilm formation has a clearly established role during systemic Candida infections, it is not known whether C. albicans forms biofilms on the vaginal mucosa and the possible role of biofilms in disease. In vivo and ex vivo murine vaginitis models were employed to examine biofilm formation by scanning electron and confocal microscopy. C. albicans strains included 3153A (lab strain), DAY185 (parental control strain), and mutants defective in morphogenesis and/or biofilm formation in vitro (efg1/efg1 and bcr1/bcr1). Both 3153A and DAY815 formed biofilms on the vaginal mucosa in vivo and ex vivo as indicated by high fungal burden and microscopic analysis demonstrating typical biofilm architecture and presence of extracellular matrix (ECM) co-localized with the presence of fungi. In contrast, efg1/efg1 and bcr1/bcr1 mutant strains exhibited weak or no biofilm formation/ECM production in both models compared to wild-type strains and complemented mutants despite comparable colonization levels. These data show for the first time that C. albicans forms biofilms in vivo on vaginal epithelium, and that in vivo biotic biofilm formation requires regulators of biofilm formation (BCR1) and morphogenesis (EFG1).
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影响因子：
9.8
作者：
Bauters, TGM;Dhont, MA;Nelis, HJ
通讯作者：
Nelis, HJ
影响因子：
--
作者：
Norice, Carmelle T.;Smith, Frank J., Jr.;Mitchell, Aaron P.
通讯作者：
Mitchell, Aaron P.
影响因子：
64.5
作者：
Lo, HJ;Kohler, JR;Fink, GR
通讯作者：
Fink, GR
影响因子：
9.2
作者：
Nobile, CJ;Mitchell, AP
通讯作者：
Mitchell, AP
影响因子：
3.1
作者：
Davis, D;Edwards, JE;Ibrahim, AS
通讯作者：
Ibrahim, AS