Riboproteome remodeling during quiescence exit in Saccharomyces cerevisiae.
Riboproteome remodeling during quiescence exit in Saccharomyces cerevisiae.
复制标题
酿酒酵母休眠期核糖蛋白质组重构。
DOI：
10.1016/j.isci.2023.108727
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发表时间：
2024-01-19
期刊：
影响因子：
5.8
通讯作者：
Portela, Paula
中科院分区：
文献类型：
--
作者：
Solari, Clara A.;Martinez, Maria Clara Ortola;Fernandez, Juan M.;Bates, Christian;Cueto, Gerardo;Valacco, Maria Pia;Morales-Polanco, Fabian;Moreno, Silvia;Rossi, Silvia;Ashe, Mark P.;Portela, Paula
静止状态是大多数细胞中细胞生命的普遍模式。酿酒酵母是研究细胞周期、静止和衰老的分子基础的有用模型。以往的研究表明，异质性核糖体在特定的刺激下表现出特殊的翻译功能，以调节细胞蛋白质组。使用纳米LC-MS/MS，我们确定了69的79个核糖体蛋白（RP），构成真核生物80 S核糖体在静止期。我们的研究表明，核糖蛋白质组由444个辅助蛋白组成，包括细胞功能，如翻译，蛋白质折叠，氨基酸和葡萄糖代谢，细胞对氧化应激的反应，和蛋白质降解。此外，核糖体颗粒上的RP和辅助蛋白质的化学计量是不同的，这取决于生长条件以及在单体和多聚体组分之间。不同RP的缺乏导致翻译能力的缺陷，这表明核糖体组成可以导致静止期翻译活性的变化。静止期酵母细胞含有不同组成的核糖体。在静止期，核糖体的组成在单体和多聚核糖体之间变化。核糖体的组成可导致翻译活性的变化。
The quiescent state is the prevalent mode of cellular life in most cells. Saccharomyces cerevisiae is a useful model for studying the molecular basis of the cell cycle, quiescence, and aging. Previous studies indicate that heterogeneous ribosomes show a specialized translation function to adjust the cellular proteome upon a specific stimulus. Using nano LC-MS/MS, we identified 69 of the 79 ribosomal proteins (RPs) that constitute the eukaryotic 80S ribosome during quiescence. Our study shows that the riboproteome is composed of 444 accessory proteins comprising cellular functions such as translation, protein folding, amino acid and glucose metabolism, cellular responses to oxidative stress, and protein degradation. Furthermore, the stoichiometry of both RPs and accessory proteins on ribosome particles is different depending on growth conditions and among monosome and polysome fractions. Deficiency of different RPs resulted in defects of translational capacity, suggesting that ribosome composition can result in changes in translational activity during quiescence. Quiescent yeast cells contain ribosomes with different compositions The ribosome composition varies between monosomes and polysomes during quiescence exit Ribosome composition can result in changes in translational activity Cell biology
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影响因子：
3.9
作者：
Boria, Ilenia;Garelli, Emanuela;Gazda, Hanna T.;Aspesi, Anna;Quarello, Paola;Pavesi, Elisa;Ferrante, Daniela;Meerpohl, Joerg J.;Kartal, Mutlu;Da Costa, Lydie;Proust, Alexis;Leblanc, Thierry;Simansour, Maud;Dahl, Niklas;Froejmark, Anne-Sophie;Pospisilova, Dagmar;Cmejla, Radek;Beggs, Alan H.;Sheen, Mee R.;Landowski, Michael;Buros, Christopher M.;Clinton, Catherine M.;Dobson, Lori J.;Vlachos, Adrianna;Atsidaftos, Eva;Lipton, Jeffrey M.;Ellis, Steven R.;Ramenghi, Ugo;Dianzani, Irma
通讯作者：
Dianzani, Irma
影响因子：
2
作者：
Giardina BJ;Stanley BA;Chiang HL
通讯作者：
Chiang HL
影响因子：
4.1
作者：
Joo, Minju;Yeom, Ji-Hyun;Choi, Younkyung;Jun, Hyeon;Song, Wooseok;Kim, Hyun-Lee;Lee, Kangseok;Shin, Eunkyoung
通讯作者：
Shin, Eunkyoung
影响因子：
5.6
作者：
Schmitt K;Kraft AA;Valerius O
通讯作者：
Valerius O
影响因子：
3.2
作者：
FUGE, EK;BRAUN, EL;WERNERWASHBURNE, M
通讯作者：
WERNERWASHBURNE, M