Spatial organization of chromosomes in the salivary gland nuclei of Drosophila melanogaster.
Spatial organization of chromosomes in the salivary gland nuclei of Drosophila melanogaster.
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DOI：
10.1083/jcb.102.1.112
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发表时间：
1986-01
影响因子：
7.8
通讯作者：
SEDAT, JW
中科院分区：
文献类型：
--
作者：
HOCHSTRASSER, M;MATHOG, D;GRUENBAUM, Y;SAUMWEBER, H;SEDAT, JW
利用基于计算机的模型构建和分析系统，从光学或物理切片的腺体构建了24个黑腹果蝇唾液腺细胞核的三维模型，从而对这些细胞核中的染色体折叠和包装得出了一些一般性结论。首先且最令人惊讶的是，染色体的显著卷曲具有很强的手性，以右手螺旋为主。其次，某些基因座与核膜之间的高频并置几乎完全出现在插入异染色质的位置；此外，染色中心总是与核膜并置。第三，染色体总是分离成相互排斥的空间区域，同时通常以极化（拉布尔）方向横跨细胞核延伸。第四，每条常染色体的臂几乎总是并列的，但没有其他相对臂的位置受到强烈青睐。最后，尽管存在这些非随机的结构特征，但发现每条染色体都折叠成多种不同的构象。此外，对一组细胞核进行了分析，在这些细胞核中，染色体通常聚集的着丝粒区域彼此相距很远。这些细胞核具有在正常细胞核中看到的相同结构模式。这意味着像独立的染色体区域和特定的染色体 - 核膜接触等特征在很大程度上与细胞核内不同染色体的相对位置无关。
Using a computer-based system for model building and analysis, three- dimensional models of 24 Drosophila melanogaster salivary gland nuclei have been constructed from optically or physically sectioned glands, allowing several generalizations about chromosome folding and packaging in these nuclei. First and most surprising, the prominent coiling of the chromosomes is strongly chiral, with right-handed gyres predominating. Second, high frequency appositions between certain loci and the nuclear envelope appear almost exclusively at positions of intercalary heterochromatin; in addition, the chromocenter is always apposed to the envelope. Third, chromosomes are invariably separated into mutually exclusive spatial domains while usually extending across the nucleus in a polarized (Rabl) orientation. Fourth, the arms of each autosome are almost always juxtaposed, but no other relative arm positions are strongly favored. Finally, despite these nonrandom structural features, each chromosome is found to fold into a wide variety of different configurations. In addition, a set of nuclei has been analyzed in which the normally aggregrated centromeric regions of the chromosomes are located far apart from one another. These nuclei have the same architectural motifs seen in normal nuclei. This implies that such characteristics as separate chromosome domains and specific chromosome-nuclear envelope contacts are largely independent of the relative placement of the different chromosomes within the nucleus.
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影响因子：
3.1
作者：
Bridges, CB
通讯作者：
Bridges, CB
DOI：
10.1016/0006-291x(70)90786-2
发表时间：
1970-01-01
影响因子：
3.1
作者：
BURGOYNE, LA;WAGAR, MA;ATKINSON, MR
通讯作者：
ATKINSON, MR
影响因子：
1.6
作者：
FUSSELL, CP
通讯作者：
FUSSELL, CP
影响因子：
2
作者：
MATHOG, D
通讯作者：
MATHOG, D
影响因子：
1.6
作者：
OHNUKI, Y
通讯作者：
OHNUKI, Y