The immature human ovary shows loss of abnormal follicles and increasing follicle developmental competence through childhood and adolescence.
The immature human ovary shows loss of abnormal follicles and increasing follicle developmental competence through childhood and adolescence.
复制标题
DOI：
10.1093/humrep/det388
复制
发表时间：
2014-01
期刊：
影响因子：
--
通讯作者：
Telfer EE
中科院分区：
文献类型：
--
作者：
Anderson RA;McLaughlin M;Wallace WH;Albertini DF;Telfer EE
儿童和青少年的卵泡在形态和体外生长潜力上与成人的卵泡不同吗？青春期前卵巢中含有高比例的形态异常的非生长卵泡，卵泡的体外生长能力降低。已知青春期前卵巢在早期生长阶段含有卵泡。这种情况在儿童期和青春期如何变化尚不清楚，也没有关于青春期前和青春期女孩卵泡体外生长潜力的先前数据。对5名青春期前和7名青春期女孩以及19名成年妇女的卵巢活检进行组织学分析，体外培养6天，然后分离生长卵泡并再培养6天。卵巢活检标本取自接受卵巢组织冷冻保存以保存生育能力的女孩，并与成年妇女的活检标本进行比较。对卵泡发育阶段和形态进行了分类。培养6天后，评估卵泡生长起始。在另外6天内评估分离的次级卵泡的生长，包括卵母细胞生长的分析。青春期前卵巢中含有高比例的异常非生长卵泡（19.4%对青春期卵巢中的4.85%;分析了4004个卵泡; P = 0.02），其特征在于不清楚的生殖囊泡膜和核仁缺失。这种异常形态的卵泡在成年卵巢中未见。在6天培养期间，在所有年龄段均观察到卵泡生长启动;在青春期前样本中，几乎没有发育到第二阶段，而青春期样本显示出与成人组织中观察到的相似的生长激活（青春期组：P = 0.02 vs青春期前，NS vs成人）。将分离的次级卵泡再培养6天。那些来自青春期前卵巢的卵泡显示出有限的生长（与青春期和成年卵泡相比P < 0.05），并且在此期间卵母细胞直径没有变化。来自青春期卵巢的卵泡显示出增长;与来自成年女性的卵泡相比，这仍然是减少的（P < 0.05），但卵母细胞生长与卵泡大小成比例。这些数据仅来自少量卵巢活检，尽管分析了大量卵泡。目前还不清楚群体之间的差异是否与青春期有关，或者只是年龄。这些发现表明，所有年龄段女孩的卵泡都可以在体外诱导生长，这对一些卵巢组织恶性污染风险高的患者具有重要意义。孤立卵泡的生长减少表明，除了局部环境的潜在差异外，还存在真正的卵泡内差异，并且在儿童期和青春期卵巢中存在成熟过程，这涉及异常卵泡的丢失和卵泡发育能力的增加。由MRC赠款G 0901839和G1100357资助。没有竞争利益。
Do the ovarian follicles of children and adolescents differ in their morphology and in vitro growth potential from those of adults? Pre-pubertal ovaries contained a high proportion of morphologically abnormal non-growing follicles, and follicles showed reduced capacity for in vitro growth. The pre-pubertal ovary is known to contain follicles at the early growing stages. How this changes over childhood and through puberty is unknown, and there are no previous data on the in vitro growth potential of follicles from pre-pubertal and pubertal girls. Ovarian biopsies from five pre-pubertal and seven pubertal girls and 19 adult women were analysed histologically, cultured in vitro for 6 days, with growing follicles then isolated and cultured for a further 6 days. Ovarian biopsies were obtained from girls undergoing ovarian tissue cryopreservation for fertility preservation, and compared with biopsies from adult women. Follicle stage and morphology were classified. After 6 days in culture, follicle growth initiation was assessed. The growth of isolated secondary follicles was assessed over a further 6 days, including analysis of oocyte growth. Pre-pubertal ovaries contained a high proportion of abnormal non-growing follicles (19.4 versus 4.85% in pubertal ovaries; 4004 follicles analysed; P = 0.02) characterized by indistinct germinal vesicle membrane and absent nucleolus. Follicles with this abnormal morphology were not seen in the adult ovary. During 6 days culture, follicle growth initiation was observed at all ages; in pre-pubertal samples there was very little development to secondary stages, while pubertal samples showed similar growth activation to that seen in adult tissue (pubertal group: P = 0.02 versus pre-pubertal, ns versus adult). Isolated secondary follicles were cultured for a further 6 days. Those from pre-pubertal ovary showed limited growth (P < 0.05 versus both pubertal and adult follicles) and no change in oocyte diameter over that period. Follicles from pubertal ovaries showed increased growth; this was still reduced compared with follicles from adult women (P < 0.05) but oocyte growth was proportionate to follicle size. These data derive from only a small number of ovarian biopsies, although large numbers of follicles were analysed. It is unclear whether the differences between groups are related to puberty, or just age. These findings show that follicles from girls of all ages can be induced to grow in vitro, which has important implications for some patients who are at high risk of malignant contamination of their ovarian tissue. The reduced growth of isolated follicles indicates that there are true intrafollicular differences in addition to potential differences in their local environment, and that there are maturational processes occurring in the ovary through childhood and adolescence, which involve the loss of abnormal follicles, and increasing follicle developmental competence. Funded by MRC grants G0901839 and G1100357. No competing interests.
登录
查看更多内容
影响因子：
5.8
作者：
Fulton, N;da Silva, SJM;Anderson, RA
通讯作者：
Anderson, RA
影响因子：
8.4
作者：
Ernst, Erik;Kjaersgaard, Mimi;Andersen, Claus Yding
通讯作者：
Andersen, Claus Yding
影响因子：
3.6
作者：
Mork, Lindsey;Maatouk, Danielle M.;Capel, Blanche
通讯作者：
Capel, Blanche
影响因子：
13.3
作者：
Jadoul, Pascale;Dolmans, Marie-Madeleine;Donnez, Jacques
通讯作者：
Donnez, Jacques
影响因子：
8.8
作者：
Himelstein-Braw, R;Peters, H;Faber, M
通讯作者：
Faber, M