Polymeric ionically conductive composite matrices and electrical stimulation strategies for nerve regeneration: In vitro characterization.
Polymeric ionically conductive composite matrices and electrical stimulation strategies for nerve regeneration: In vitro characterization.
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聚合物电离导电复合矩阵和神经再生的电刺激策略：体外表征。
DOI：
10.1002/jbm.b.34272
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发表时间：
2019-08
期刊：
影响因子：
--
通讯作者：
Kumbar SG
中科院分区：
文献类型：
--
作者：
Manoukian OS;Stratton S;Arul MR;Moskow J;Sardashti N;Yu X;Rudraiah S;Kumbar SG
干细胞策略和电刺激（ES）的使用代表了周围神经再生的新领域。采用壳聚糖和磺化聚苯胺、木质素磺酸盐等离子导电聚合物包覆静电纺聚己内酯/醋酸纤维素微纳米纤维，制备复合基体。这些复合基质具有表面形态、涂层均匀性、离子电导率和机械强度等特征，可以作为神经再生支架材料与电刺激相结合。复合基体的电导率在0.0049-0.0068 mS/m之间，这是由于在微纳米纤维上均匀涂覆了磺化聚合物。将植入人间充质干细胞（hMSCs）的IC聚合物薄膜（2D）和复合纤维基质（3D）以0.5V， 20Hz电流刺激1小时，连续刺激14天，研究细胞活力、形态和神经元样表型表达的变化。体外ES处理组和阳性对照生长因子处理组的hMSCs成纤维细胞形态改变为带细胞体的细长神经突样结构。免疫荧光染色显示，在ES反应中存在神经元标记物，包括β3-微管蛋白、微管相关蛋白2和巢蛋白。
Stem cell strategies and the use of electrical stimulation (ES) represent promising new frontiers for peripheral nerve regeneration. Composite matrices were fabricated by coating electrospun polycaprolactone/cellulose acetate micro-nanofibers with chitosan and ionically-conductive (IC) polymers including, sulfonated polyaniline and lignin sulfonate. These composite matrices were characterized for surface morphology, coating uniformity, ionic conductivity, and mechanical strength to explore as scaffold materials for nerve regeneration in conjunction with electrical stimulation. Composite matrices measured conductivity in the range of 0.0049–0.0068 mS/m due to the uniform coating of sulfonated polymers on the micro-nanofibers. Thin films (2D) and composite fiber matrices (3D) of IC polymers seeded with human mesenchymal stem cells (hMSCs) were electrically stimulated at 0.5V, 20Hz for 1h daily for 14 days to study the changes in cell viability, morphology, and expression of the neuronal-like phenotype. In vitro ES lead to changes in hMSCs’ fibroblast morphology into elongated neurite-like structures with cell bodies for ES-treated and positive control growth factor-treated groups. Immunofluorescent staining revealed the presence of neuronal markers including β3-tubulin, microtubule-associated protein 2, and nestin in response to ES.
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周围神经再生策略：电刺激聚合物基于聚合物的神经生长导管。
影响因子：
--
作者：
Anderson M;Shelke NB;Manoukian OS;Yu X;McCullough LD;Kumbar SG
通讯作者：
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影响因子：
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Keating CP
影响因子：
3.9
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通讯作者：
Reis, Rui L.