Fabrication of Self-Cleaning, Reusable Titania Templates for Nanometer and Micrometer Scale Protein Patterning.
Fabrication of Self-Cleaning, Reusable Titania Templates for Nanometer and Micrometer Scale Protein Patterning.
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用于纳米和微米级蛋白质图案化的自清洁、可重复使用的二氧化钛模板的制造。
作者：
Mark Moxey;Alexander Johnson;O. El;M. Cartron;S. S. Dinachali;C. Hunter;M. Saifullah;K. Chong;G. Leggett
二氧化钛的光催化自清洁特性有助于制备可重复使用的蛋白质纳米颗粒模板。采用干涉光刻(IL)和纳米压印光刻(NIL)技术在平方厘米的面积上制备了二氧化钛纳米结构。利用劳埃德镜双光束干涉仪，利用光催化纳米光刻技术在钛薄膜的天然氧化物表面形成了烷基膦酸盐自组装单分子膜。在干涉图中暴露在最大值的区域，通过光催化氧化去除单层。在暴露于最低强度的区域，单层保持完好。曝光后，样品在食人鱼溶液中被刻蚀，得到宽度为30 nm的钛纳米结构。采用硅压印法制备了二氧化钛树脂旋转膜，经过焙烧和反应离子刻蚀后，形成了纳米二氧化钛。对于这两种制备技术，随后对低聚(乙二醇)功能化的三氯硅烷的吸附产生了完全被动的、抗蛋白质的表面。近紫外光照射通过光催化降解使这种抗蛋白质的薄膜从二氧化钛区域移除，使二氧化硅区域上的钝化硅烷膜完好无损。用荧光染料标记的蛋白质被吸附到二氧化钛区域，得到具有明亮荧光的纳米分子。随后对样品进行近紫外光照射，通过光催化降解将蛋白质从二氧化钛纳米结构中移除，从而促进了不同蛋白质的吸附。这个过程被重复了多次。这些简单的方法似乎产生了耐用的、可重复使用的样品，对于需要纳米结构生物接口但无法获得纳米制造所需基础设施的实验室来说，这些样品可能很有价值。
The photocatalytic self-cleaning characteristics of titania facilitate the fabrication of reuseable templates for protein nanopatterning. Titania nanostructures were fabricated over square centimeter areas by interferometric lithography (IL) and nanoimprint lithography (NIL). With the use of a Lloyd's mirror two-beam interferometer, self-assembled monolayers of alkylphosphonates adsorbed on the native oxide of a Ti film were patterned by photocatalytic nanolithography. In regions exposed to a maximum in the interferogram, the monolayer was removed by photocatalytic oxidation. In regions exposed to an intensity minimum, the monolayer remained intact. After exposure, the sample was etched in piranha solution to yield Ti nanostructures with widths as small as 30 nm. NIL was performed by using a silicon stamp to imprint a spin-cast film of titanium dioxide resin; after calcination and reactive ion etching, TiO2 nanopillars were formed. For both fabrication techniques, subsequent adsorption of an oligo(ethylene glycol) functionalized trichlorosilane yielded an entirely passive, protein-resistant surface. Near-UV exposure caused removal of this protein-resistant film from the titania regions by photocatalytic degradation, leaving the passivating silane film intact on the silicon dioxide regions. Proteins labeled with fluorescent dyes were adsorbed to the titanium dioxide regions, yielding nanopatterns with bright fluorescence. Subsequent near-UV irradiation of the samples removed the protein from the titanium dioxide nanostructures by photocatalytic degradation facilitating the adsorption of a different protein. The process was repeated multiple times. These simple methods appear to yield durable, reuseable samples that may be of value to laboratories that require nanostructured biological interfaces but do not have access to the infrastructure required for nanofabrication.
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影响因子：
15
作者：
Alswieleh, Abdullah M.;Cheng, Nan;Canton, Irene;Ustbas, Burcin;Xue, Xuan;Ladmiral, Vincent;Xia, Sijing;Ducker, Robert E.;El Zubir, Osama;Cartron, Michael L.;Hunter, C. Neil;Leggett, Graham J.;Armes, Steven P.
通讯作者：
Armes, Steven P.
影响因子：
56.9
作者：
Chen, CS;Mrksich, M;Ingber, DE
通讯作者：
Ingber, DE
影响因子：
10.8
作者：
Ahijado-Guzman, Ruben;Prasad, Janak;Soennichsen, Carsten
通讯作者：
Soennichsen, Carsten
影响因子：
15
作者：
Ahmad, Shahrul A. Alang;Wong, Lu Shin;Micklefield, Jason
通讯作者：
Micklefield, Jason
影响因子：
2.1
作者：
Brown TD;Slotosch A;Thibaudeau L;Taubenberger A;Loessner D;Vaquette C;Dalton PD;Hutmacher DW
通讯作者：
Hutmacher DW