Bioconversion of farnesol and 1,4-dihydroxy-2-naphthoate to menaquinone by an immobilized whole-cell biocatalyst using engineered Elizabethkingia meningoseptica
Bioconversion of farnesol and 1,4-dihydroxy-2-naphthoate to menaquinone by an immobilized whole-cell biocatalyst using engineered Elizabethkingia meningoseptica
复制标题
使用工程化的 Elizabethkingia meningoseptica 通过固定化全细胞生物催化剂将金合欢醇和 1,4-二羟基-2-萘甲酸酯生物转化为甲基萘醌
DOI：
10.1007/s11274-017-2382-7
复制
发表时间：
2017-11
期刊：
影响因子：
--
通讯作者：
Su Yun
中科院分区：
文献类型：
--
作者：
Liu Yan;Yang Ziming;Xue Zhenglian;Wang Zhou;Zhao Shiguang;Zhu Longbao;Hu Liuxiu;Ding Xiumin;Su Yun
菜籽酮(MK)在医药和食品工业中有着重要的应用。为了提高MK-4的产量(QP)，我们利用基因工程脑膜败血伊丽莎白的全细胞，建立了一种直接从其前体1，4-二羟基-2-萘酸酯(DHNA)和法尼醇合成MK-4的生物转化方法。结果表明，无论是用游离的FM-D198细胞还是固定化的FM-D198细胞，法尼醇和DHNA都可以直接合成MK-4。在两相转化体系中，MK-4的产率在有机相达到29.85 ± 0.36 mg/gDCW，12h达到最高值24.08 ± 0.33 mg/gDCW。固定化细胞培养8h，MK-4产量达到26.34 ± 1.35 mg/gDCW和17.44 ± 1.05 mg/gDCW。尽管固定化细胞的产量低于游离细胞，但通过重复分批培养，固定化细胞可以重复用于MK-4的生产。经过10次分批培养，MK-4产量保持在20 mg/L以上。优化催化体系后，固定化细胞的MK-4产量达到26.91 ± 1.27 mg/L，DHNA和法尼醇的摩尔转化率分别为58.56%和76.90%。
Menaquinone (MK) has important applications in the pharmaceutical and food industries. To increase the production rate (QP) of MK-4, we developed a straightforward biotransformation method for MK-4 synthesis directly from its precursors 1,4-dihydroxy-2-naphthoate (DHNA) and farnesol using whole cells of genetically engineered Elizabethkingia meningoseptica. Results showed that MK-4 can be produced directly from farnesol and DHNA using both free and immobilized FM-D198 cells. MK-4 yield peaked at 29.85 ± 0.36 mg/L in the organic phase and 24.08 ± 0.33 mg/g DCW after 12 h of bioconversion using free cells in a two-phase conversion system. MK-4 yield reached 26.34 ± 1.35 mg/L and 17.44 ± 1.05 mg/g DCW after 8 h using immobilized cells. Although this yield was lower than that using free cells, immobilized cells can be re-used for MK-4 production via repeated-batch culture. After ten batch cultures, efficient MK-4 production was maintained at a yield of more than 20 mg/L. After optimizing the catalysis system, the MK-4 yield reached 26.91 ± 1.27 mg/L using the immobilized cells and had molar conversion rates of 58.56 and 76.90% for DHNA and farnesol, respectively.
登录
查看更多内容
DOI：
10.1271/bbb1961.53.3017
发表时间：
1989-11
期刊：
Agricultural and biological chemistry
影响因子：
--
作者：
H. Taguchi;T. Shibata;C. Duangmanee;Y. Tani
通讯作者：
H. Taguchi;T. Shibata;C. Duangmanee;Y. Tani
影响因子：
2.4
作者：
Liu Yan;Zhiming Zheng;Hongwei Qiu;Genhai Zhao;W. Peng;Liu Hui;Wang Li;Zhe-min Li;Hefang Wu
通讯作者：
Liu Yan;Zhiming Zheng;Hongwei Qiu;Genhai Zhao;W. Peng;Liu Hui;Wang Li;Zhe-min Li;Hefang Wu
影响因子：
64.8
作者：
Nakagawa, Kimie;Hirota, Yoshihisa;Okano, Toshio
通讯作者：
Okano, Toshio
影响因子：
5
作者：
Ebrahiminezhad, Alireza;Varma, Vikas;Berenjian, Aydin
通讯作者：
Berenjian, Aydin
影响因子：
1
作者：
M. Tan;Hui Liu;Zhe-min Li;Xiaowen Sun;Zhiming Zheng;Genhai Zhao
通讯作者：
M. Tan;Hui Liu;Zhe-min Li;Xiaowen Sun;Zhiming Zheng;Genhai Zhao