Role of a class DHC1b dynein in retrograde transport of IFT motors and IFT raft particles along cilia, but not dendrites, in chemosensory neurons of living Caenorhabditis elegans.
Role of a class DHC1b dynein in retrograde transport of IFT motors and IFT raft particles along cilia, but not dendrites, in chemosensory neurons of living Caenorhabditis elegans.
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DOI：
10.1083/jcb.147.3.519
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发表时间：
1999-11-01
期刊：
影响因子：
--
通讯作者：
Scholey JM
中科院分区：
文献类型：
--
作者：
Signor D;Wedaman KP;Orozco JT;Dwyer ND;Bargmann CI;Rose LS;Scholey JM
使用基于荧光显微镜的转运测定，可以观察到异源三聚体运动蛋白、驱动蛋白-II及其推定的货物在活的秀丽隐杆线虫的化学感觉神经元的感觉纤毛内通过鞭毛内转运（IFT）以0.7 μm/s顺行移动（Orozco，J.T.，K.P. Wedaman，D.先生，H。布朗湖，澳-地罗斯和J.M. Scholey 1999.自然398：674）。在这里，我们报告驱动蛋白-II，和它的两个假定的货物分子，OSM-1和OSM-6，都在逆行方向沿着纤毛和树突，这是一致的假设，这些蛋白质从纤毛的远端回收通过逆行运输途径，使它们沿着纤毛，然后树突，回到神经元细胞体。为了检验负末端导向微管马达蛋白，细胞质动力蛋白，驱动这一逆行运输途径的假设，我们可视化运动的驱动蛋白-II和它的货物沿着树突和纤毛在一个che-3细胞质动力蛋白突变体的背景，并观察到在纤毛，但不是在树突逆行运输的抑制。相反，顺行IFT在che-3突变体中正常进行。因此，我们建议DHC 1b类细胞质动力蛋白，CHE-3，是专门负责逆行运输的顺行电机，驱动蛋白-II，其货物内的感觉纤毛，但不是树突。
The heterotrimeric motor protein, kinesin-II, and its presumptive cargo, can be observed moving anterogradely at 0.7 μm/s by intraflagellar transport (IFT) within sensory cilia of chemosensory neurons of living Caenorhabditis elegans, using a fluorescence microscope–based transport assay (Orozco, J.T., K.P. Wedaman, D. Signor, H. Brown, L. Rose, and J.M. Scholey. 1999. Nature. 398:674). Here, we report that kinesin-II, and two of its presumptive cargo molecules, OSM-1 and OSM-6, all move at ∼1.1 μm/s in the retrograde direction along cilia and dendrites, which is consistent with the hypothesis that these proteins are retrieved from the distal endings of the cilia by a retrograde transport pathway that moves them along cilia and then dendrites, back to the neuronal cell body. To test the hypothesis that the minus end–directed microtubule motor protein, cytoplasmic dynein, drives this retrograde transport pathway, we visualized movement of kinesin-II and its cargo along dendrites and cilia in a che-3 cytoplasmic dynein mutant background, and observed an inhibition of retrograde transport in cilia but not in dendrites. In contrast, anterograde IFT proceeds normally in che-3 mutants. Thus, we propose that the class DHC1b cytoplasmic dynein, CHE-3, is specifically responsible for the retrograde transport of the anterograde motor, kinesin-II, and its cargo within sensory cilia, but not within dendrites.
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影响因子：
2.7
作者：
HEDGECOCK, EM;CULOTTI, JG;PERKINS, LA
通讯作者：
PERKINS, LA
影响因子：
11.4
作者：
FIRE, A
通讯作者：
FIRE, A
DOI：
10.1083/jcb.141.4.993
发表时间：
1998-05-18
期刊：
The Journal of cell biology
影响因子：
--
作者：
Cole DG;Diener DR;Himelblau AL;Beech PL;Fuster JC;Rosenbaum JL
通讯作者：
Rosenbaum JL
DOI：
10.1083/jcb.91.3.661
发表时间：
1981-12
期刊：
The Journal of cell biology
影响因子：
--
作者：
Heidemann SR;Landers JM;Hamborg MA
通讯作者：
Hamborg MA
影响因子：
7.8
作者：
KOZMINSKI, KG;BEECH, PL;ROSENBAUM, JL
通讯作者：
ROSENBAUM, JL