Genomic analysis of the core components of ABA signaling reveals their possible role in abiotic stress response in cassava
Genomic analysis of the core components of ABA signaling reveals their possible role in abiotic stress response in cassava
复制标题
ABA 信号核心成分的基因组分析揭示了它们在木薯非生物胁迫反应中的可能作用
DOI：
10.1016/j.envexpbot.2019.103855
复制
发表时间：
2019-11
影响因子：
5.7
通讯作者：
Hu Wei
中科院分区：
文献类型：
--
作者：
Zhao Hui;Wu Chunlai;Yan Yan;Tie Weiwei;Ding Zehong;Liu Guanghua;Yan Wei;Li Yanan;Wang Wenquan;Peng Ming;Guo Jianchun;Guo Anping;Hu Wei
脱落酸（阿坝）信号在植物发育和对环境刺激的响应中起着重要作用。目前，作为阿坝信号转导核心组分的PYL-PP 2C-SnRK 2家族在木薯中的研究还不够深入。在这项研究中，我们确定了13个PYLs，80 PP 2Cs，和10 SnRK 2木薯基因组，支持的进化关系，蛋白质基序，和基因结构分析。一般来说，大多数PYL-PP 2C-SnRK 2在Arg 7和W14之间的不同组织中具有相似的表达谱。比较表达分析表明，野生亚种W14的MePP 2Cs活性低于栽培品种Arg 7和SC 124，这可能是W14较强耐性的原因。相互作用网络和共表达分析表明，较少的A组PP 2C介导的网络表现出的转录变化，在W14的叶和根比在Arg 7。几个选择的PYL-PP 2C-SnRK 2基因的表达表明，阿坝和多种非生物胁迫显着上调。综上所述，本研究为PYL-PP 2C-SnRK 2介导的木薯干旱适应在转录水平上提供了新的见解，并确定了一些作物对非生物胁迫耐受性遗传改良的候选者。
Abscisic acid (ABA) signaling plays an important role in plants development and response to environmental stimuli. Currently, the PYL-PP2C-SnRK2 families, as the core components of ABA signaling, are not well understood in cassava, a drought tolerant crop. In this study, we identified 13 PYLs, 80 PP2Cs, and 10 SnRK2s from the cassava genome, supported by evolutionary relationship, protein motif, and gene structure analyses. Generally, most of thePYL-PP2C-SnRK2shad similar expression profiles in different tissues between Arg7 and W14. Comparative expression analyses showed less activation ofMePP2Csin wild subspecies W14, compared with cultivated varieties Arg7 and SC124, which may contributed to the strong tolerance of W14. Interaction networks and co-expression assays demonstrated that less Group A PP2C-mediated network showed transcriptional changes in leaves and roots of W14 than that in Arg7. Expression of several selectedPYL-PP2C-SnRK2genes indicated their significant upregulation upon ABA and multiple abiotic stresses. Taken together, this study provides new insights intoPYL-PP2C-SnRK2-mediated drought adaption at transcription levels in cassava and identifies some candidates for genetic improvement of crop tolerance to abiotic stress.
登录
查看更多内容
影响因子：
4.4
作者：
Cao J;Jiang M;Li P;Chu Z
通讯作者：
Chu Z
影响因子：
2.9
作者：
Krzywińska E;Kulik A;Bucholc M;Fernandez MA;Rodriguez PL;Dobrowolska G
通讯作者：
Dobrowolska G
DOI：
10.1126/science.1173041
发表时间：
2009-05-22
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Park SY;Fung P;Nishimura N;Jensen DR;Fujii H;Zhao Y;Lumba S;Santiago J;Rodrigues A;Chow TF;Alfred SE;Bonetta D;Finkelstein R;Provart NJ;Desveaux D;Rodriguez PL;McCourt P;Zhu JK;Schroeder JI;Volkman BF;Cutler SR
通讯作者：
Cutler SR
影响因子：
3.7
作者：
Mou W;Li D;Bu J;Jiang Y;Khan ZU;Luo Z;Mao L;Ying T
通讯作者：
Ying T
杨树SnRK2基因异源过度表达增强拟南芥盐胁迫耐受性
影响因子：
5.6
作者：
Song X;Yu X;Hori C;Demura T;Ohtani M;Zhuge Q
通讯作者：
Zhuge Q