Retinal Degeneration in a Murine Model of Retinal Ischemia by Unilateral Common Carotid Artery Occlusion.
Retinal Degeneration in a Murine Model of Retinal Ischemia by Unilateral Common Carotid Artery Occlusion.
复制标题
单侧颈总动脉闭塞致视网膜缺血模型的视网膜变性研究。
DOI：
10.1155/2021/7727648
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发表时间：
2021
影响因子：
--
通讯作者：
Kurihara T
中科院分区：
文献类型：
--
作者：
Lee D;Nakai A;Miwa Y;Tomita Y;Serizawa N;Katada Y;Hatanaka Y;Tsubota K;Negishi K;Kurihara T
视网膜变性是眼部血管疾病中视网膜的进行性损害。这有几个原因，如动脉或静脉阻塞，糖尿病视网膜病变，或遗传性视网膜疾病。为了研究视网膜退行性变的病理机制，需要建立具有实验重复性和临床相关性的模型。在我们以前的研究中，我们建立了一种模拟人类眼缺血综合征(OIS)的单侧颈总动脉阻塞(UCCAO)的小鼠视网膜低灌流模型，并描述了UCCAO后视网膜的广泛病理机制。然而，UCCAO的眼部病理过程仍有缺失的部分。在这项研究中，我们研究了这些未被发现的机制。成年小鼠行双侧颈总动脉结扎。与假手术小鼠相比，UCCAO后检查了眼功能障碍、组织学缺陷和炎症反应。采用电生理学、组织学和分子生物学等方法对评价值进行分析。UCCAO术后永久性眼睑下垂。麻醉下急性可逆性白内障的诱导时间点缩短。UCCAO后2~4周检测到视网膜/视觉功能障碍。具体地说，暗视b波比a波受到的影响更大，明视b波功能障碍。持续观察受累的振荡电位和视觉诱发电位。以神经节细胞层Neu阳性细胞丢失为特征的病理性Müler胶质细胞增生症/炎症反应。UCCAO对眼轴、眼压、瞳孔光反射、视网膜色素上皮/脉络膜厚度无明显影响。UCCAO制作的小鼠视网膜缺血模型可用于研究缺血视网膜的一系列变性过程。
Retinal degeneration is a progressive retinal damage in ocular vascular diseases. There are several reasons for this, such as occlusion of arteries or veins, diabetic retinopathy, or hereditary retinal diseases. To study pathological mechanisms of retinal degeneration, it is required to develop experimentally reproducible and clinically relevant models. In our previous studies, we developed a murine model of retinal hypoperfusion by unilateral common carotid artery occlusion (UCCAO) which mimics the pathophysiology of ocular ischemic syndrome (OIS) in humans, and described broad pathological mechanisms in the retina after UCCAO. However, there still remain missing pieces of the ocular pathologic process by UCCAO. In this study, we examined those unfound mechanisms. UCCAO was performed on adult mice. Ocular dysfunctions, histological deficits, and inflammation were examined after UCCAO, compared with sham-operated mice. Evaluation values were analyzed by electrophysiological, histological, and molecular biological methods. Eyelid drooping was permanently seen after UCCAO. Induction time point of acute reversible cataract under anesthesia was shortened. Retinal/visual dysfunctions were detected 2-4 weeks after UCCAO. Specifically, scotopic b-wave was more affected than a-wave, with the dysfunction of photopic b-wave. Impaired oscillatory potentials and visual evoked potential were constantly observed. Pathological Müller gliosis/inflammation was featured with NeuN-positive cell loss in the ganglion cell layer. Axial length, intraocular pressure, pupillary light reflex, and retinal pigment epithelium/choroidal thickness were not changed by UCCAO. A murine model of retinal ischemia by UCCAO can be useful for studying a series of degenerative process in the ischemic retina.
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DOI：
10.1007/s10633-020-09770-3
发表时间：
2021-03
期刊：
Documenta ophthalmologica. Advances in ophthalmology
影响因子：
--
作者：
Hamilton R;Bach M;Heinrich SP;Hoffmann MB;Odom JV;McCulloch DL;Thompson DA
通讯作者：
Thompson DA
影响因子：
1.9
作者：
Dong, CJ;Agey, P;Hare, WA
通讯作者：
Hare, WA
影响因子：
2
作者：
Bermudez, Maria A.;Vicente, Ana F.;Gonzalez, Francisco
通讯作者：
Gonzalez, Francisco
影响因子：
3.7
作者：
Hattori Y;Kitamura A;Nagatsuka K;Ihara M
通讯作者：
Ihara M
影响因子：
6.3
作者：
BARONE, FC;KNUDSEN, DJ;WILLETTE, RN
通讯作者：
WILLETTE, RN