High-speed label-free functional photoacoustic microscopy of mouse brain in action.
High-speed label-free functional photoacoustic microscopy of mouse brain in action.
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小鼠脑的高速无标记功能光学显微镜在作用中。
DOI:
10.1038/nmeth.3336
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发表时间:
2015-05
期刊:
影响因子:
48
通讯作者:
Wang, Lihong V.
中科院分区:
文献类型:
--
作者:
Yao, Junjie;Wang, Lidai;Yang, Joon-Mo;Maslov, Konstantin I.;Wong, Terence T. W.;Li, Lei;Huang, Chih-Hsien;Zou, Jun;Wang, Lihong V.
We present fast functional photoacoustic microscopy (PAM), which is capable of three-dimensional high-resolution high-speed imaging of the mouse brain, complementary to other imaging modalities. A single-wavelength pulse-width-based method was implemented to image blood oxygenation with capillary-level resolution and a one-dimensional imaging rate of 100 kHz. We applied PAM to image the vascular morphology, blood oxygenation, blood flow, and oxygen metabolism in the brain in both resting and stimulated states.
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