High-speed label-free functional photoacoustic microscopy of mouse brain in action.

High-speed label-free functional photoacoustic microscopy of mouse brain in action.
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小鼠脑的高速无标记功能光学显微镜在作用中。

DOI:
10.1038/nmeth.3336
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发表时间:
2015-05
期刊:
影响因子:
48
通讯作者:
Wang, Lihong V.
Wang, Lihong V.
中科院分区:
生物学1区
文献类型:
--
作者:
Yao, Junjie;Wang, Lidai;Yang, Joon-Mo;Maslov, Konstantin I.;Wong, Terence T. W.;Li, Lei;Huang, Chih-Hsien;Zou, Jun;Wang, Lihong V.

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我们提出了快速功能光声显微镜(PAM),这是能够三维高分辨率高速成像的小鼠大脑,补充其他成像方式。一个单波长脉冲宽度为基础的方法实现了图像的血液氧合与毛细血管水平的分辨率和一维成像速率为100 kHz。我们应用PAM成像的血管形态,血氧,血流,和氧代谢在大脑中的休息和刺激状态。
We present fast functional photoacoustic microscopy (PAM), which is capable of three-dimensional high-resolution high-speed imaging of the mouse brain, complementary to other imaging modalities. A single-wavelength pulse-width-based method was implemented to image blood oxygenation with capillary-level resolution and a one-dimensional imaging rate of 100 kHz. We applied PAM to image the vascular morphology, blood oxygenation, blood flow, and oxygen metabolism in the brain in both resting and stimulated states.
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