Modulation of ovine SBD-1 expression by 17beta-estradiol in ovine oviduct epithelial cells.
Modulation of ovine SBD-1 expression by 17beta-estradiol in ovine oviduct epithelial cells.
复制标题
17β-雌二醇对绵羊输卵管上皮细胞中绵羊SBD-1表达的调节
DOI：
10.1186/1746-6148-8-143
复制
发表时间：
2012-08-25
影响因子：
2.6
通讯作者：
Wuriliga
中科院分区：
文献类型：
--
作者：
Wen S;Cao G;Bao T;Cheng L;Li H;Du C;Tu Y;Li Q;Jian R;Zhao P;Wuriliga
背景粘膜上皮，包括输卵管的粘膜上皮，分泌抗菌先天免疫分子（AIIMS）。它们对多种病原体具有杀菌/抑菌功能。在 AIIM 中，羊 β-防御素-1 (SBD-1) 是最有效的之一。尽管SBD-1是一种重要的AIIM并且受到雌激素的密切调节，但17β-雌二醇的调节机制尚未明确。我们使用逆转录定量 PCR (RT-qPCR) 研究了 E2 和激动剂或抑制剂对绵羊输卵管上皮细胞 SBD-1 表达的影响。另外,分别或同时抑制3条不同的通路,以证实不同抑制剂在调控机制中的作用。结果17β-雌二醇(E2)诱导绵羊输卵管上皮细胞释放SBD-1。 SBD-1 表达是通过绵羊输卵管上皮细胞中 G 蛋白偶联受体 30 (GPR30) 和雌激素受体 (ER) 激活介导的。抑制蛋白激酶A（PKA）、蛋白激酶C（PKC）和活化B细胞核因子κ轻链增强子（NF-κB）的基因表达导致SBD-1表达降低。结论综上所述，在绵羊输卵管上皮细胞中，E2诱导的SBD-1表达的上调在前期依赖于GPR30，在后期依赖于ERs，我们假设该作用是通过以下方式完成的： PKA、PKC 和 NF-κB 通路同时进行。
BackgroundMucosal epithelia, including those of the oviduct, secrete antimicrobial innate immune molecules (AIIMS). These have bactericidal/bacteriostatic functions against a variety of pathogens. Among the AIIMs, sheep β-defensin-1 (SBD-1) is one of the most potent. Even though the SBD-1 is an important AIIM and it is regulated closely by estrogenic hormone, the regulation mechanism of 17β-estradiol has not been clearly established. We investigated the effects of E2and agonist or inhibitor on ovine oviduct epithelial cells in regard to SBD-1 expression using reverse transcription quantitative PCR (RT-qPCR). In addition, three different pathways were inhibited separately or simultaneously to confirm the effect of different inhibitors in the regulation mechanism.Results17beta-estradiol (E2) induced release of SBD-1 in ovine oviduct epithelial cells. SBD-1 expression was mediated through G-protein-coupled receptor 30 (GPR30) and Estrogen Receptors (ERs) activation in ovine oviduct epithelial cell. Inhibition of gene expression of protein kinase A (PKA), protein kinase C (PKC), and nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) led to a decreased SBD-1 expression.ConclusionsTaken together, E2-induced up-regulation of SBD-1 expressions were GPR30-dependent during prophase and ERs-dependent during later-stage in ovine oviduct epithelial cells, and we assume that the effect was completed by the PKA, PKC, and NF-κB pathways simultaneous.
登录
查看更多内容
DOI：
10.1006/bbrc.1997.7734
发表时间：
1997-11-26
影响因子：
3.1
作者：
Takada, Y;Kato, C;Ando, J
通讯作者：
Ando, J
影响因子：
--
作者：
New, David C;Wong, Yung H
通讯作者：
Wong, Yung H
影响因子：
10.4
作者：
Yin XJ;Schafer R;Ma JY;Antonini JM;Weissman DD;Siegel PD;Barger MW;Roberts JR;Ma JK
通讯作者：
Ma JK
影响因子：
16.8
作者：
Yang, D;Biragyn, A;Oppenheim, JJ
通讯作者：
Oppenheim, JJ
DOI：
10.1016/0165-5876(80)90007-5
发表时间：
1980-01-01
影响因子：
1.5
作者：
MOGI, G;MAEDA, S;WATANABE, N
通讯作者：
WATANABE, N