Distinct types of fibrocyte can differentiate from mononuclear cells in the presence and absence of serum.
Distinct types of fibrocyte can differentiate from mononuclear cells in the presence and absence of serum.
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DOI：
10.1371/journal.pone.0009730
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发表时间：
2010-03-18
期刊：
影响因子：
3.7
通讯作者：
Salmon M
中科院分区：
文献类型：
--
作者：
Curnow SJ;Fairclough M;Schmutz C;Kissane S;Denniston AK;Nash K;Buckley CD;Lord JM;Salmon M
纤维细胞是骨髓来源的细胞，表达造血和基质细胞标志物，其有助于组织修复以及病理性纤维化。纤维细胞的分化仍然缺乏特征，这限制了对其生物学和功能的理解。特别地，使用两种方法在体外产生纤维细胞，其根本上区别在于血清的存在或不存在。我们在这里表明，在没有血清（SF）的情况下生长的纤维细胞比CD14+单核细胞更有效地从外周血单核细胞分化，并通过失去其梭形纤维细胞形态对血清作出反应。虽然在血清（SC）存在下产生的纤维细胞表达相同范围的标志物，但它们更有效地从CD14+单核细胞分化，并且响应于血清不改变它们的形态。转录分析表明，这两种类型的纤维细胞是彼此不同的，成纤维细胞和额外的单核细胞衍生的后代。SF和SC纤维细胞之间显著不同的基因途径包括涉及细胞迁移、免疫应答和对创伤的应答的基因途径。这些数据表明，SF和SC纤维细胞是不同的，但相关的细胞类型，并表明它们将在组织修复和纤维化过程中发挥不同的作用，其中血清蛋白的变化可能会发生。
Fibrocytes are bone-marrow derived cells, expressing both haematopoietic and stromal cell markers, which contribute to tissue repair as well as pathological fibrosis. The differentiation of fibrocytes remains poorly characterised and this has limited understanding of their biology and function. In particular two methods are used to generate fibrocytes in vitro that differ fundamentally by the presence or absence of serum. We show here that fibrocytes grown in the absence of serum (SF) differentiate more efficiently from peripheral blood mononuclear cells than CD14+ monocytes, and respond to serum by losing their spindle-shaped fibrocyte morphology. Although fibrocytes generated in the presence of serum (SC) express the same range of markers, they differentiate more efficiently from CD14+ monocytes and do not change their morphology in response to serum. Transcriptional analysis revealed that both types of fibrocyte are distinct from each other, fibroblasts and additional monocyte-derived progeny. The gene pathways that differ significantly between SF and SC fibrocytes include those involved in cell migration, immune responses and response to wounding. These data show that SF and SC fibrocytes are distinct but related cell types, and suggest that they will play different roles during tissue repair and fibrosis where changes in serum proteins may occur.
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DOI：
10.1016/j.biocel.2006.11.005
发表时间：
2007-01-01
影响因子：
4
作者：
McAnulty, Robin J.
通讯作者：
McAnulty, Robin J.
DOI：
10.1073/pnas.94.12.6307
发表时间：
1997-06-10
影响因子：
11.1
作者：
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通讯作者：
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影响因子：
5
作者：
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通讯作者：
Bucala, Richard
影响因子：
3.1
作者：
Uehara, Hirotsugu;Nakagawa, Tomoo;Saito, Yasushi
通讯作者：
Saito, Yasushi
影响因子：
15.9
作者：
Phillips, RJ;Burdick, MD;Strieter, RM
通讯作者：
Strieter, RM