Postanesthetic effects of isoflurane on behavioral phenotypes of adult male C57BL/6J mice.
Postanesthetic effects of isoflurane on behavioral phenotypes of adult male C57BL/6J mice.
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DOI：
10.1371/journal.pone.0122118
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Goto T
中科院分区：
文献类型：
--
作者：
Yonezaki K;Uchimoto K;Miyazaki T;Asakura A;Kobayashi A;Takase K;Goto T
异氟烷以前是主要的临床麻醉剂，但现在主要用于兽医麻醉。研究报告了异氟烷的广泛作用部位，表明除镇静作用外，还有广泛的副作用。在本研究中，我们对异氟烷处理的小鼠进行表型分析，以研究异氟烷的麻醉后行为效应。我们应用综合行为测试组合，包括感觉测试组合、运动测试组合、焦虑测试组合、抑郁测试组合、社交测试组合、注意测试组合和学习测试组合，在1.8%异氟醚麻醉后7天开始。除对照组外，我们还纳入了一个轭式对照组，在麻醉前暴露于与异氟烷给药动物相同的处理应激。我们的综合行为测试组合显示异氟烷治疗组的潜伏抑制受损，但脑中残留异氟烷的浓度可能可以忽略不计。对照组和异氟醚治疗组在高架十字迷宫测试中表现出较高的焦虑，在线索恐惧条件反射测试中表现出学习功能受损。在感觉功能、运动功能、抗抑郁行为和社会行为方面没有观察到影响。异氟烷对动物行为的影响已有文献报道，但尚未进行系统研究。据我们所知，这项研究是第一项系统研究成年后暴露于异氟烷的小鼠的一般健康状况、神经反射、感觉功能、运动功能和高级行为功能的研究。我们的研究结果表明，异氟醚麻醉后效应导致注意缺陷的小鼠。因此，在临床环境和兽医麻醉中必须非常小心地使用异氟烷。
Isoflurane was previously the major clinical anesthetic agent but is now mainly used for veterinary anesthesia. Studies have reported widespread sites of action of isoflurane, suggesting a wide array of side effects besides sedation. In the present study, we phenotyped isoflurane-treated mice to investigate the postanesthetic behavioral effects of isoflurane. We applied comprehensive behavioral test batteries comprising sensory test battery, motor test battery, anxiety test battery, depression test battery, sociability test battery, attention test battery, and learning test battery, which were started 7 days after anesthesia with 1.8% isoflurane. In addition to the control group, we included a yoked control group that was exposed to the same stress of handling as the isoflurane-treated animals before being anesthetized. Our comprehensive behavioral test batteries revealed impaired latent inhibition in the isoflurane-treated group, but the concentration of residual isoflurane in the brain was presumably negligible. The yoked control group and isoflurane-treated group exhibited higher anxiety in the elevated plus-maze test and impaired learning function in the cued fear conditioning test. No influences were observed in sensory functions, motor functions, antidepressant behaviors, and social behaviors. A number of papers have reported an effect of isoflurane on animal behaviors, but no systematic investigation has been performed. To the best of our knowledge, this study is the first to systematically investigate the general health, neurological reflexes, sensory functions, motor functions, and higher behavioral functions of mice exposed to isoflurane as adults. Our results suggest that the postanesthetic effect of isoflurane causes attention deficit in mice. Therefore, isoflurane must be used with great care in the clinical setting and veterinary anesthesia.
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影响因子：
2
作者：
Harrell, AV;Allan, AM
通讯作者：
Allan, AM
影响因子：
2.1
作者：
Fidalgo, A. R.;Cibelli, M.;Ma, D.
通讯作者：
Ma, D.
影响因子：
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作者：
Lee, Sunam;Park, Sang-Hon;Zuo, Zhiyi
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Zuo, Zhiyi
影响因子：
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作者：
HENRY, KR;WILLOTT, JF
通讯作者：
WILLOTT, JF
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作者：
Matsuo N;Tanda K;Nakanishi K;Yamasaki N;Toyama K;Takao K;Takeshima H;Miyakawa T
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Miyakawa T