Perfusion-based co-culture model system for bone tissue engineering.
Perfusion-based co-culture model system for bone tissue engineering.
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DOI：
10.3934/bioeng.2020009
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发表时间：
2020
影响因子：
2.3
通讯作者：
Soman P
中科院分区：
文献类型：
--
作者：
Sawyer SW;Zhang K;Horton JA;Soman P
在这项工作中，我们报告了一种基于灌注的共培养系统，可用于骨组织工程应用。该模型系统是使用原代人脐静脉内皮细胞（HUVECs）和成骨细胞样Saos-2细胞的组合创建的，这些细胞被包裹在凝胶甲基丙烯酸酯(GelMA)-胶原水凝胶混合物中，包含在3D打印的可灌注结构中。该结构包含双通道，在一个定制的生物反应器中，用成骨介质灌注长达两周，以诱导矿物质沉积。在仅含有huvec、Saos-2细胞或两者组合的构建体中，通过微ct量化矿物质沉积，以确定内皮细胞和骨样细胞的组合是否增加了矿物质沉积。组织学和荧光染色用于验证沿灌注通道和通道之间的矿物质沉积和细胞功能。虽然仅Saos-2与Saos-2 + HUVEC样品中沉积的矿物数量没有可量化的差异，但沉积矿物的位置在两组之间存在显着差异，这表明在GelMA基质中添加HUVEC允许Saos-2细胞在构建的扩散限制区域沉积骨矿物。这项工作作为如何使用3D打印和细胞共培养的组合创建可灌注骨组织工程结构的模型。
In this work, we report on a perfusion-based co-culture system that could be used for bone tissue engineering applications. The model system is created using a combination of Primary Human Umbilical Vein Endothelial Cells (HUVECs) and osteoblast-like Saos-2 cells encapsulated within a Gelatin Methacrylate (GelMA)-collagen hydrogel blend contained within 3D printed, perfusable constructs. The constructs contain dual channels, within a custom-built bioreactor, that were perfused with osteogenic media for up to two weeks in order to induce mineral deposition. Mineral deposition in constructs containing only HUVECs, only Saos-2 cells, or a combination thereof was quantified by microCT to determine if the combination of endothelial cells and bone-like cells increased mineral deposition. Histological and fluorescent staining was used to verify mineral deposition and cellular function both along and between the perfused channels. While there was not a quantifiable difference in the amount of mineral deposited in Saos-2 only versus Saos-2 plus HUVEC samples, the location of the deposited mineral differed dramatically between the groups and indicated that the addition of HUVECs within the GelMA matrix allowed Saos-2 cells, in diffusion limited regions of the construct, to deposit bone mineral. This work serves as a model on how to create perfusable bone tissue engineering constructs using a combination of 3D printing and cellular co-cultures.
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影响因子：
2.8
作者：
Lee, Vivian K.;Lanzi, Alison M.;Ngo, Haygan;Yoo, Seung-Schik;Vincent, Peter A.;Dai, Guohao
通讯作者：
Dai, Guohao
影响因子：
3.1
作者：
Albrecht, Lucas D.;Sawyer, Stephen W.;Soman, Pranav
通讯作者：
Soman, Pranav
成骨细胞和内皮细胞共培养：培养基和细胞比例的优化
影响因子：
3
作者：
Ma, Jinling;van den Beucken, Jeroen J. J. P.;Jansen, John A.
通讯作者：
Jansen, John A.
影响因子：
41.2
作者：
通讯作者：
--
影响因子：
19
作者：
Cuchiara, Michael P.;Gould, Daniel J.;McHale, Melissa K.;Dickinson, Mary E.;West, Jennifer L.
通讯作者：
West, Jennifer L.