Effects of Buffalo Milk and Cow Milk on Lipid Metabolism in Obese Mice Induced by High Fat.
Effects of Buffalo Milk and Cow Milk on Lipid Metabolism in Obese Mice Induced by High Fat.
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DOI：
10.3389/fnut.2022.841800
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发表时间：
2022
影响因子：
5
通讯作者：
Zhao, Guoqi
中科院分区：
文献类型：
--
作者：
Jiang, Maocheng;Meng, Zitong;Cheng, Zhiqiang;Zhan, Kang;Ma, Xiaoyu;Yang, Tianyu;Huang, Yinghao;Yan, Qi;Gong, Xiaoxiao;Zhao, Guoqi
本研究的目的是评价水牛奶和牛奶对肥胖小鼠脂质代谢的影响。牛奶成分分析表明，水牛奶中的脂肪、蛋白质和总固形物含量高于牛奶，而乳糖含量低于牛奶。经乳代谢物提取和LC-MS/MS分析，差异代谢物主要富集于“亚油酸代谢途径”、“戊糖与葡萄糖酸相互转化途径”和“细胞色素P450代谢外源物质途径”。实验采用三组C57BL/6J小鼠，每组6只，连续饲养5周：(1)高脂饲料组（HFD组）；(2)高脂饮食+水牛奶组（HBM组）；(3)高脂日粮+牛奶组（HCM组）。我们的研究结果显示，与HFD组相比，HBM和HCM组小鼠的体重在1 ~ 4周内明显降低。与HFD和HBM组相比，HCM组肝脏组织中参与脂质代谢的ACAA2、ACACB和SLC27A5基因mRNA表达量显著升高。H&E染色及油红O染色结果显示，HCM组大鼠肝脏脂肪细胞数量、大小及脂肪堆积均较HFD组明显减少，而HBM组无明显变化。与HFD和HCM组相比，HBM组TNF-α和IL-1β炎症基因mRNA水平显著升高，这与结肠组织切片炎性细胞浸润和组织破坏的结果一致。由此可见，饲粮中添加牛奶对肥胖小鼠的减肥和脂质代谢具有有益作用。
The aim of this study was to evaluate the effects of buffalo milk and cow milk on lipid metabolism in obese mice. Milk composition analysis showed fat, protein, and total solid content in buffalo milk was higher than cow milk, while the lactose content of buffalo milk was lower than cow milk. After milk metabolite extraction and LC-MS/MS analysis, differential metabolites were mainly enriched in “linoleic acid metabolism pathways,” “pentose and glucuronate interconversion pathways,” and “metabolism of xenobiotics by cytochrome P450 pathways.” We fed three groups of C57BL/6J mice (n = 6 per group) for 5 weeks: (1) high-fat diet group (HFD group); (2) high-fat diet + buffalo milk group (HBM group); and (3) high-fat diet + cow milk group (HCM group). Our results showed that body weight of mice was significantly decreased in HBM and HCM groups from 1 to 4 weeks compared with the HFD group. The mRNA expression of ACAA2, ACACB, and SLC27A5 genes involved in the lipid metabolism in liver tissue were significantly elevated in HCM group, relatively to HFD and HBM group. In addition, the adipocyte number, size and lipid accumulation in the liver were significantly decreased in HCM group compared with the HFD group by H&E staining and oil red O staining, but was not change in HBM group. The mRNA levels of TNF-α and IL-1β inflammatory genes were significantly increased in HBM group, relatively to HFD and HCM group, which is consistent with results from inflammatory cell infiltration and tissue disruption by colon tissue sections. In conclusion, dietary supplementation of cow milk has beneficial effects on loss of weight and lipid metabolism in obese mice.
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影响因子：
3.6
作者：
Lorenzen, Janne K.;Astrup, Arne
通讯作者：
Astrup, Arne
影响因子：
4.4
作者：
Chen, Qixuan;Reimer, Raylene A.
通讯作者：
Reimer, Raylene A.
Fpr2 缺乏可通过减少体重增加和抑制巨噬细胞趋化性和 M1 极化介导的炎症来减轻饮食引起的胰岛素抵抗
影响因子：
7.7
作者：
Chen, Xiaofang;Zhuo, Shu;Le, Yingying
通讯作者：
Le, Yingying
影响因子：
3.3
作者：
Huguet, A.;Le Dividich, J.;Le Huerou-Luron, I.
通讯作者：
Le Huerou-Luron, I.
影响因子：
4.5
作者：
Kwon, Hyuk Tae;Lee, Cheol Min;Cho, BeLong
通讯作者：
Cho, BeLong