Plasmodium simium, a Plasmodium vivax-related malaria parasite: genetic variability of Duffy binding protein II and the Duffy antigen/receptor for chemokines.
Plasmodium simium, a Plasmodium vivax-related malaria parasite: genetic variability of Duffy binding protein II and the Duffy antigen/receptor for chemokines.
复制标题
疟原虫，疟原虫相关的疟疾寄生虫：达菲结合蛋白II的遗传变异性和趋化因子的Duffy抗原/受体。
DOI：
10.1371/journal.pone.0131339
复制
发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Ferreira Alves de Brito C
中科院分区：
文献类型：
--
作者：
Camargos Costa D;Pereira de Assis GM;de Souza Silva FA;Araújo FC;de Souza Junior JC;Braga Hirano ZM;Satiko Kano F;Nóbrega de Sousa T;Carvalho LH;Ferreira Alves de Brito C
西姆疟原虫是一种来自新大陆猴子的寄生虫，与人类疟疾寄生虫间日疟原虫关系最近；它也自然感染人类。间日疟原虫血液期感染依赖于Duffy结合蛋白II(PvDBPII)及其在红细胞上的同源受体，即趋化因子Duffy抗原受体(HDARC)，但尚无关于间日疟原虫红细胞入侵途径的信息。由于西伯利亚原虫可能也依赖于DBPII/DARC的相互作用，因此从自然感染西伯利亚原虫的南方褐吼猴(Alouatta Guariba Clitans)中获得了编码西伯利亚原虫DBP(PsDBPII)和猿猴DARC(SDARC)的基因序列。间日疟原虫和间日疟原虫的DBP结合区序列高度相似。但PsDBPII的遗传变异低于PvDBPII。系统发育分析表明，这些基因具有严格的亲缘关系，聚在进化树的同一分支中。测序结果表明，该基因含有三个新的非同义替换。这些替换都没有位于DARC的N-末端结构域，该结构域直接与DBPII相互作用。通过COS7细胞表面表达PvDBPII的细胞黏附实验评价sDARC与PvDBPII之间的相互作用。体外抑制结合实验表明，猴血清抗体阻断了表达PvDBPII的COS-7细胞与hDARC阳性红细胞之间的相互作用。综上所述，系统发育分析强化了宿主从人类到猴子的转换可能发生在最近的进化过程中的假设，这有助于揭示新大陆疟原虫的进化史。进一步的侵袭研究将证实西门氏菌是否依赖于DBP/DARC来触发内化到红细胞中。
Plasmodium simium is a parasite from New World monkeys that is most closely related to the human malaria parasite Plasmodium vivax; it also naturally infects humans. The blood-stage infection of P. vivax depends on Duffy binding protein II (PvDBPII) and its cognate receptor on erythrocytes, the Duffy antigen receptor for chemokines (hDARC), but there is no information on the P. simium erythrocytic invasion pathway. The genes encoding P. simium DBP (PsDBPII) and simian DARC (sDARC) were sequenced from Southern brown howler monkeys (Alouatta guariba clamitans) naturally infected with P. simium because P. simium may also depend on the DBPII/DARC interaction. The sequences of DBP binding domains from P. vivax and P. simium were highly similar. However, the genetic variability of PsDBPII was lower than that of PvDBPII. Phylogenetic analyses demonstrated that these genes were strictly related and clustered in the same clade of the evolutionary tree. DARC from A. clamitans was also sequenced and contained three new non-synonymous substitutions. None of these substitutions were located in the N-terminal domain of DARC, which interacts directly with DBPII. The interaction between sDARC and PvDBPII was evaluated using a cytoadherence assay of COS7 cells expressing PvDBPII on their surfaces. Inhibitory binding assays in vitro demonstrated that antibodies from monkey sera blocked the interaction between COS-7 cells expressing PvDBPII and hDARC-positive erythrocytes. Taken together, phylogenetic analyses reinforced the hypothesis that the host switch from humans to monkeys may have occurred very recently in evolution, which sheds light on the evolutionary history of new world plasmodia. Further invasion studies would confirm whether P. simium depends on DBP/DARC to trigger internalization into red blood cells.
登录
查看更多内容
影响因子：
3.7
作者：
Culleton R;Coban C;Zeyrek FY;Cravo P;Kaneko A;Randrianarivelojosia M;Andrianaranjaka V;Kano S;Farnert A;Arez AP;Sharp PM;Carter R;Tanabe K
通讯作者：
Tanabe K
影响因子：
3.7
作者：
Ceravolo, I. P.;Souza-Silva, F. A.;Carvalho, L. H.
通讯作者：
Carvalho, L. H.
影响因子：
2.8
作者：
Curado, I;Duarte, AMRC;Kloetzel, JK
通讯作者：
Kloetzel, JK
影响因子：
3.7
作者：
Chootong, Patchanee;Panichakul, Tasanee;Adams, John H.
通讯作者：
Adams, John H.
影响因子：
4.6
作者：
Ceravolo, I. P.;Sanchez, B. A. M.;Carvalho, L. H.
通讯作者：
Carvalho, L. H.