Peptidoglycan crosslinking relaxation promotes Helicobacter pylori's helical shape and stomach colonization.
Peptidoglycan crosslinking relaxation promotes Helicobacter pylori's helical shape and stomach colonization.
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DOI：
10.1016/j.cell.2010.03.046
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发表时间：
2010-05-28
期刊：
影响因子：
64.5
通讯作者：
Salama NR
中科院分区：
文献类型：
--
作者：
Sycuro LK;Pincus Z;Gutierrez KD;Biboy J;Stern CA;Vollmer W;Salama NR
细菌细胞产生螺旋细胞形状的机制及其功能作用知之甚少。人类病原体幽门螺杆菌的螺旋形状可能有助于穿透其复制的厚胃粘液。我们确定了四个螺旋形状所需的基因：三个新的LytM肽聚糖内肽酶同源物（csd 1 -3）和一个CCMA同源物。大多数细菌的胞质膜周围的肽聚糖（胞壁蛋白）小囊是由肽交联连接的聚糖链组成的网状结构。来自缺乏任何单一csd基因或ccmA的突变体的完整细胞和分离的球囊形成弯曲的杆，并显示出增加的肽聚糖交联。多基因缺失突变体的定量形态学分析显示，每个蛋白质独特的形状生成途径。尽管胃具有正常的定向运动性，但该途径是胃的稳健定殖所必需的。我们的研究结果表明，多种蛋白质的协调作用放松肽聚糖交联，使螺旋细胞的曲率和扭曲。
The mechanisms by which bacterial cells generate helical cell shape and its functional role are poorly understood. Helical shape of the human pathogen Helicobacter pylori may facilitate penetration of the thick gastric mucus where it replicates. We identified four genes required for helical shape: three novel LytM peptidoglycan endopeptidase homologues (csd1–3) and a ccmA homologue. Surrounding the cytoplasmic membrane of most bacteria, the peptidoglycan (murein) sacculus is a meshwork of glycan strands joined by peptide cross-links. Intact cells and isolated sacculi from mutants lacking any single csd gene or ccmA formed curved rods and showed increased peptidoglycan cross-linking. Quantitative morphological analyses of multiple-gene deletion mutants revealed each protein uniquely contributes to a shape-generating pathway. This pathway is required for robust colonization of the stomach in spite of normal directional motility. Our findings suggest that the coordinated action of multiple proteins relaxes peptidoglycan cross-linking, enabling helical cell curvature and twist.
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影响因子：
3.1
作者：
Eaton, KA;Suerbaum, S;Krakowka, S
通讯作者：
Krakowka, S
DOI：
10.1073/pnas.0805309105
发表时间：
2008-12-09
影响因子：
11.1
作者：
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通讯作者：
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影响因子：
6.4
作者：
HAZELL, SL;LEE, A;HENNESSY, W
通讯作者：
HENNESSY, W
影响因子：
11.4
作者：
Cabeen, Matthew T.;Charbon, Godefroid;Jacobs-Wagner, Christine
通讯作者：
Jacobs-Wagner, Christine
影响因子：
29.4
作者：
Cover TL;Blaser MJ
通讯作者：
Blaser MJ