Heritability of biting time behaviours in the major African malaria vector Anopheles arabiensis.
Heritability of biting time behaviours in the major African malaria vector Anopheles arabiensis.
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DOI：
10.1186/s12936-023-04671-7
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发表时间：
2023-08-16
期刊：
影响因子：
3
通讯作者：
Ferguson, Heather M.
中科院分区：
文献类型：
--
作者：
Govella, Nicodem J.;Johnson, Paul C. D.;Killeen, Gerry F.;Ferguson, Heather M.
使用经杀虫剂处理的蚊帐控制疟疾与蚊子媒介取食行为的变化有关，包括对人类的早期叮咬和户外叮咬。表型可塑性和遗传性对这些行为变化的相对贡献尚不清楚。阐明这些变化背后的机制对于预测对病媒控制的影响至关重要。使用一种新型便携式半场系统(PSFS)对坦桑尼亚的疟疾媒介阿拉伯按蚊的叮咬时间遗传力进行了实验测量。狂野的安。用人类登陆捕获法(HLC)每小时采集一次的阿拉比虫，根据捕获时间分为3类：早期(18：00-21：00)、中期(22：00-04：00)和晚期(05：00-07：00)，并分别放在单独的笼子里。然后给蚊子喂食血粉以产卵和形成第一代子代(F1)。分别饲养各叮咬时间表型类别的F1代，并与其母亲同时采血捕捉寻找寄主。得到的卵子被用来产生F2代，用于遗传力分析。遗传力通过释放F2An进行评估。阿拉比斯进入PSFS，记录它们在人类落地捕获期间的咬合时间，并与它们的F0祖母的时间进行比较。在PSFS分析中，F2代后代的叮咬时间(早：18：00-21：00，中：22：00-04：00或晚：05：00-07：00)与野生捕获的F0代祖母的叮咬时间显著正相关，相应的估计遗传度为0.110(95%CI 0.003，0.208)。早咬F0的F2比中、晚咬F0的F2更有可能早咬。同样，来自中晚咬F0的F2比早咬F0产生的晚咬的概率更高。尽管遗传力不大，但我们的结果表明，咬合时间的一些变化可归因于加性遗传差异。因此，选择可以有效地控制蚊子叮咬的时间，突出了针对早期和户外叮咬蚊子的控制方法的必要性。网上版载有补充材料，可在10.1186/s12936-023-04671-7查阅。
The use of insecticide-treated nets for malaria control has been associated with shifts in mosquito vector feeding behaviour including earlier and outdoor biting on humans. The relative contribution of phenotypic plasticity and heritability to these behavioural shifts is unknown. Elucidation of the mechanisms behind these shifts is crucial for anticipating impacts on vector control. A novel portable semi-field system (PSFS) was used to experimentally measure heritability of biting time in the malaria vector Anopheles arabiensis in Tanzania. Wild An. arabiensis from hourly collections using the human landing catch (HLC) method were grouped into one of 3 categories based on their time of capture: early (18:00–21:00), mid (22:00–04:00), and late (05:00–07:00) biting, and placed in separate holding cages. Mosquitoes were then provided with a blood meal for egg production and formation of first filial generation (F1). The F1 generation of each biting time phenotype category was reared separately, and blood fed at the same time as their mothers were captured host-seeking. The resultant eggs were used to generate the F2 generation for use in heritability assays. Heritability was assessed by releasing F2 An. arabiensis into the PSFS, recording their biting time during a human landing catch and comparing it to that of their F0 grandmothers. In PSFS assays, the biting time of F2 offspring (early: 18:00–21:00, mid: 22:00–04:00 or late: 05:00–07:00) was significantly positively associated with that of their wild-caught F0 grandmothers, corresponding to an estimated heritability of 0.110 (95% CI 0.003, 0.208). F2 from early-biting F0 were more likely to bite early than F2 from mid or late-biting F0. Similarly, the probability of biting late was higher in F2 derived from mid and late-biting F0 than from early-biting F0. Despite modest heritability, our results suggest that some of the variation in biting time is attributable to additive genetic variation. Selection can, therefore, act efficiently on mosquito biting times, highlighting the need for control methods that target early and outdoor biting mosquitoes. The online version contains supplementary material available at 10.1186/s12936-023-04671-7.
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影响因子：
3.2
作者：
Maliti DV;Marsden CD;Main BJ;Govella NJ;Yamasaki Y;Collier TC;Kreppel K;Chiu JC;Lanzaro GC;Ferguson HM;Lee Y
通讯作者：
Lee Y
影响因子：
7.7
作者：
Huho, Bernadette;Briet, Olivier;Killeen, Gerry
通讯作者：
Killeen, Gerry
DOI：
10.4269/ajtmh.2009.09-0124
发表时间：
2009-12-01
影响因子：
3.3
作者：
Lefevre, Thierry;Gouagna, Louis-Clement;Thomas, Frederic
通讯作者：
Thomas, Frederic
影响因子：
3.5
作者：
Elanga-Ndille, Emmanuel;Nouage, Lynda;Wondji, Charles S.
通讯作者：
Wondji, Charles S.
影响因子：
3
作者：
Govella, Nicodem J.;Chaki, Prosper P.;Killeen, Gerry F.
通讯作者：
Killeen, Gerry F.