Human methanogen diversity and incidence in healthy and diseased colonic groups using mcrA gene analysis.
Human methanogen diversity and incidence in healthy and diseased colonic groups using mcrA gene analysis.
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DOI：
10.1186/1471-2180-8-79
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发表时间：
2008-05-20
期刊：
影响因子：
4.2
通讯作者：
Marchesi JR
中科院分区：
文献类型：
--
作者：
Scanlan PD;Shanahan F;Marchesi JR
人类产甲烷菌的发病率和多样性在健康和疾病的胃肠道中的特征不足。通过靶向mcrA基因的PCR和克隆文库方法，以促进调查健康和疾病组中产甲烷菌的相对发病率的双重目标，并提供人类胃肠道中产甲烷菌多样性的概述。研究了来自一组健康对照组和五个胃肠道疾病组的DNA粪便提取物（共207个）。结直肠癌、息肉切除术、肠易激综合征和对照组的产甲烷菌阳性个体数量基本相等（范围45-50%）。炎症性肠道疾病组的甲烷发生率降低，溃疡性结肠炎为24%，克罗恩病为30%。4个独特的mcrA基因的限制性片段长度多态性谱和生物信息学分析表明，大多数（94%）检索到的所有序列是100%相同的甲烷短杆菌smithii mcrA基因。此外，还回收了与口腔甲烷短杆菌和甲烷短杆菌目成员最密切相关的mcrA基因序列。mcrA基因作为一个有用的生物标志物，在人类肠道中的产甲烷菌检测和产甲烷菌发病率的变化趋势可以作为肠道功能的重要指标。尽管史氏甲烷短杆菌是健康和疾病个体的远端结肠中的优势产甲烷菌，但产甲烷菌的多样性大于先前报道的。总之，炎症性肠病中产甲烷菌的低发病率，产甲烷菌的功能和甲烷产生的影响，以及产甲烷菌与人类胃肠道中其他微生物群体之间的竞争性相互作用，值得进一步研究。
The incidence and diversity of human methanogens are insufficiently characterised in the gastrointestinal tract of both health and disease. A PCR and clone library methodology targeting the mcrA gene was adopted to facilitate the two-fold aim of surveying the relative incidence of methanogens in health and disease groups and also to provide an overview of methanogen diversity in the human gastrointestinal tract. DNA faecal extracts (207 in total) from a group of healthy controls and five gastrointestinal disease groups were investigated. Colorectal cancer, polypectomised, irritable bowel syndrome and the control group had largely equivalent numbers of individuals positive for methanogens (range 45–50%). Methanogen incidence in the inflammatory bowel disease groups was reduced, 24% for ulcerative colitis and 30% for Crohn's disease. Four unique mcrA gene restriction fragment length polymorphism profiles were identified and bioinformatic analyses revealed that the majority of all sequences (94%) retrieved from libraries were 100% identical to Methanobrevibacter smithii mcrA gene. In addition, mcrA gene sequences most closely related to Methanobrevibacter oralis and members of the order Methanosarcinales were also recovered. The mcrA gene serves as a useful biomarker for methanogen detection in the human gut and the varying trends of methanogen incidence in the human gut could serve as important indicators of intestinal function. Although Methanobrevibacter smithii is the dominant methanogen in both the distal colon of individuals in health and disease, the diversity of methanogens is greater than previously reported. In conclusion, the low incidence of methanogens in Inflammatory Bowel Disease, the functionality of the methanogens and impact of methane production in addition to competitive interactions between methanogens and other microbial groups in the human gastrointestinal tract warrants further investigation.
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影响因子：
24.5
作者：
Furrie, E
通讯作者：
Furrie, E
DOI：
10.1097/00005176-199005000-00010
发表时间：
1990-05-01
影响因子：
2.9
作者：
FIEDOREK, SC;PUMPHREY, CL;CASTEEL, HB
通讯作者：
CASTEEL, HB
DOI：
10.1073/pnas.0308766101
发表时间：
2004-04-20
影响因子：
11.1
作者：
Lepp, PW;Brinig, MM;Relman, DA
通讯作者：
Relman, DA
影响因子：
9.8
作者：
Lichtenstein, GR;Yan, SK;Hanauer, S
通讯作者：
Hanauer, S
影响因子：
2.8
作者：
MILLER, TL;WOLIN, MJ
通讯作者：
WOLIN, MJ