In vivo detection of GABA and glutamate with MEGA-PRESS: reproducibility and gender effects.
In vivo detection of GABA and glutamate with MEGA-PRESS: reproducibility and gender effects.
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带有巨型正压的GABA和谷氨酸的体内检测：可重复性和性别效应。
DOI：
10.1002/jmri.22520
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发表时间：
2011-05
影响因子：
4.4
通讯作者：
Martin, Ernst
中科院分区：
文献类型：
--
作者：
O'Gorman, Ruth L.;Michels, Lars;Edden, Richard A.;Murdoch, James B.;Martin, Ernst
评价采用3种不同光谱拟合方法获得的γ-氨基丁酸（GABA）和谷氨酸浓度的重现性，并研究神经递质水平的性别相关差异。在3特斯拉（3 T）下，从14名成年志愿者（7名女性）的背外侧前额叶皮层中的30 mL体素采集单体素MEGA编辑的PRESS MR谱。对于每个参与者，在相同的扫描会话中获得四个连续的静息光谱。使用LCModel、jMRUI和本地编写的峰拟合软件推导代谢物浓度。每种分析方法的阶段内重现性计算为GABA和Glx（谷氨酸盐+谷氨酰胺）浓度的平均变异系数（CV）。GABA和Glx的性别差异采用双尾非配对t检验进行评价。LC模型对GABA（CV 7%）和Glx（CV 6%）的重现性最好。GABA、Glx和谷氨酸浓度在男性参与者中显著较高（分别为P = 0.02、P = 0.001和P < 0.001）。GABA和谷氨酸可以使用频域光谱拟合方法（如LCModel）在体内以高再现性（CV 6-7%）定量。然而，男性和女性之间的GABA和谷氨酸浓度差异显着，这强调了性别匹配对于研究混合队列组之间神经递质浓度差异的重要性。
To evaluate the reproducibility of γ-aminobutyric acid (GABA) and glutamate concentrations derived using three different spectral fitting methods, and to investigate gender-related differences in neurotransmitter levels. Single voxel MEGA-edited PRESS MR spectra were acquired from a 30-mL voxel in the dorsolateral prefrontal cortex in 14 adult volunteers (7 female) at 3 Tesla (3T). For each participant, four consecutive resting spectra were acquired within the same scanning session. Metabolite concentrations were derived using LCModel, jMRUI, and locally written peak fitting software. The within-session reproducibility for each analysis method was calculated as the average coefficient of variation (CV) of the GABA and Glx (glutamate+glutamine) concentrations. Gender differences in GABA and Glx were evaluated using a two-tailed unpaired t-test. LCModel provided the best reproducibility for both GABA (CV 7%) and Glx (CV 6%). GABA, Glx, and glutamate concentrations were significantly higher in the male participants, (P = 0.02, P = 0.001, and P < 0.001, respectively). GABA and glutamate can be quantified in vivo with high reproducibility (CV 6–7%) using frequency-domain spectral fitting methods like LCModel. However, the GABA and glutamate concentrations vary significantly between men and women, emphasizing the importance of gender-matching for studies investigating differences in neurotransmitter concentrations between mixed-cohort groups.
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DOI：
10.1016/j.jmr.2009.11.013
发表时间：
2010-02
期刊：
Journal of magnetic resonance (San Diego, Calif. : 1997)
影响因子：
--
作者：
Kaiser LG;Marjańska M;Matson GB;Iltis I;Bush SD;Soher BJ;Mueller S;Young K
通讯作者：
Young K
影响因子：
4.4
作者：
Hancu, Ileana
通讯作者：
Hancu, Ileana
DOI：
10.1073/pnas.0900728106
发表时间：
2009-05-19
影响因子：
11.1
作者：
Muthukumaraswamy, Suresh D.;Edden, Richard A. E.;Singh, Krish D.
通讯作者：
Singh, Krish D.
影响因子：
--
作者：
Epperson, CN;Haga, K;Krystal, JH
通讯作者：
Krystal, JH
影响因子：
3.3
作者：
Pouwels, PJW;Frahm, J
通讯作者：
Frahm, J