Analysis of neuropeptide expression and localization in adult drosophila melanogaster central nervous system by affinity cell-capture mass spectrometry.
Analysis of neuropeptide expression and localization in adult drosophila melanogaster central nervous system by affinity cell-capture mass spectrometry.
复制标题
DOI：
10.1021/pr800601x
复制
发表时间：
2009-03
影响因子：
4.4
通讯作者：
Kravitz EA
中科院分区：
文献类型：
--
作者：
Yew JY;Wang Y;Barteneva N;Dikler S;Kutz-Naber KK;Li L;Kravitz EA
已经开发出一种使用质谱、一种新型神经元亲和捕获技术和果蝇基因操作的组合方法来表征成年果蝇大脑中神经肽的表达和定位。在成人整个大脑的提取物中，对来自 18 个肽家族的 42 种神经肽进行了测序。还对目标细胞群进行了神经肽分析，这些细胞群使用遗传表达标记通过免疫亲和纯化进行富集。
A combined approach using mass spectrometry, a novel neuron affinity capture technique, and Drosophila melanogaster genetic manipulation has been developed to characterize the expression and localization of neuropeptides in the adult D. melanogaster brain. In extract from the whole adult brain, 42 neuropeptides from 18 peptide families were sequenced. Neuropeptide profiling also was performed on targeted populations of cells which were enriched with immunoaffinity purification using a genetically expressed marker.
登录
查看更多内容
影响因子：
9.8
作者：
Grönke S;Müller G;Hirsch J;Fellert S;Andreou A;Haase T;Jäckle H;Kühnlein RP
通讯作者：
Kühnlein RP
影响因子：
3
作者：
Duttlinger, A;Berry, K;Nichols, R
通讯作者：
Nichols, R
影响因子：
3.6
作者：
Choi, MY;Rafaeli, A;Jurenka, RA
通讯作者：
Jurenka, RA
影响因子：
2.3
作者：
Baggerman, G;Boonen, K;Schoofs, L
通讯作者：
Schoofs, L
影响因子：
30.8
作者：
Dierick, Herman A.;Greenspan, Ralph J.
通讯作者：
Greenspan, Ralph J.