Fine mapping and candidate gene analysis of leaf tip premature senescence and Dwarf Mutant dls-1 in Rice
Fine mapping and candidate gene analysis of leaf tip premature senescence and Dwarf Mutant dls-1 in Rice
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水稻叶尖早衰及矮化突变体dls-1精细定位及候选基因分析
DOI：
10.1007/s10725-021-00715-7
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发表时间：
2021-05
影响因子：
4.2
通讯作者：
Hu Jiang
中科院分区：
文献类型：
--
作者：
Rao Yuchun;Jiao Ran;Ye Hanfei;Hu Juan;Lu Tao;Wu Xianmei;Fang Yunxia;Li Sanfeng;Lin Han;Wang Sheng;Pan Chenyang;Lu Caolin;Wang Yuexing;Hu Jiang
水稻叶片早衰对水稻产量和品质有不利影响。鉴定早衰突变体并研究其基因功能对作物遗传改良具有重要意义。本研究利用甲基磺酸乙酯诱变粳稻云稻，获得了一个叶片衰老突变体dls-1，该突变体表现为叶片提前衰老，株高降低，穗长、结实率和千粒重等产量相关性状下降。生化分析表明，dls-1中活性氧的积累导致细胞凋亡率增加、气孔长度延长、水分散失增加和叶片硅化程度下降。转录组学数据表明，病原体抗性相关基因在dls-1中下调，导致对细菌诱导的枯萎病的敏感性增加。遗传分析表明，dls-1的突变性状受一对隐性核基因控制，并利用图位克隆技术将该基因定位于水稻第1染色体上约159 kb的区域。共发现23个开放阅读框，没有类似的表型基因的报告。RNA-seq和qRT-PCR分析表明，水稻内切-1，3-β-葡聚糖酶候选基因LOC_Os01g71670在dls-1中的表达水平与野生型相比存在显著差异。LOC_Os01g71670与大麦中的GII、水稻中的Gns 4和glu 1高度同源，参与影响植物生长发育的激素反应途径，也参与PR 2表达的调控。结合dls-1基因的表型分析，我们认为LOC_Os01g71670可能是导致叶尖早衰和抗病性丧失的候选基因。
Premature leaf senescence negatively affects rice yield and quality. Identifying premature senescence mutants and examining their gene functions are important for the genetic improvement of crops. In this study, a leaf senescence mutant dls-1 was obtained from japonica Yundao by ethyl methane sulfonate mutagenesis, which showed the phenotypes of premature leaf senescence, reduced plant height and decreased yield-associated traits including panicle length, seed setting rate, and 1000-grain weight. Biochemical analysis revealed the accumulation of reactive oxygen species in dls-1 leading to increased apoptosis rates, stomata length extension, higher water loss, and a decline in leaf silicification. Transcriptomic data showed that pathogen resistance-related genes were down-regulated in dls-1, causing increased sensitivity to bacterial-induced blight. Genetic analysis indicated that the mutant trait of dls-1 was controlled by a single recessive nuclear gene, and the gene was fine-mapped to a 159 kb region on rice chromosome 1 using map-based cloning strategy. A total of 23 open reading frames were found in the interval, and no similar phenotypic genes have been reported. However, all candidate coding genes and promoter regions were sequenced, and no mutation sites were found. RNA-seq analysis and qRT-PCR showed that the candidate gene LOC_Os01g71670, encodes a rice endo-1,3-β-glucanase, showed significantly different expression levels in dls-1 compared to the wild-type. LOC_Os01g71670 is highly homologous with GII in barley, and Gns4 and glu1 in rice, which participates in the hormone response pathway affecting plant growth and development, and also involve in the regulation of PR2 expression. Taken together with the phenotypes of dls-1, we conclude that LOC_Os01g71670 may be the candidate gene, causing the premature senescence of the leaf tip and loss of pathogen resistance.
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影响因子：
5.4
作者：
C. Li;P. Langridge;R. Lance;P. Xu;G. Fincher
通讯作者：
C. Li;P. Langridge;R. Lance;P. Xu;G. Fincher
DOI：
10.1186/s12284-021-00475-y
发表时间：
2021-04-07
期刊：
Rice (New York, N.Y.)
影响因子：
--
作者：
Yuchun RAO;Ran JIAO;Sheng WANG;Xianmei WU;Hanfei YE;Chenyang PAN;Sanfeng LI;Dedong X;Weiyong ZHOU;Gaoxing DAI;Juan HU;Deyong REN;Yuexing WANG
通讯作者：
Yuexing WANG
影响因子：
5.1
作者：
Hua-Lin Zhou;Si-Jie He;Yang Cao;Tao Chen;B. Du;C. Chu;Jin‐Song Zhang;S. Chen
通讯作者：
Hua-Lin Zhou;Si-Jie He;Yang Cao;Tao Chen;B. Du;C. Chu;Jin‐Song Zhang;S. Chen
影响因子：
6.4
作者：
Cai, Kunzheng;Gao, Dan;Zhu, Xiaoyuan
通讯作者：
Zhu, Xiaoyuan
影响因子：
4.6
作者：
Chandrima Shyam;A. Jhala;G. Kruger;M. Jugulam
通讯作者：
Chandrima Shyam;A. Jhala;G. Kruger;M. Jugulam