First report demonstrating the presence of Toxocara spp. eggs on vegetables grown in community gardens in Europe.
First report demonstrating the presence of Toxocara spp. eggs on vegetables grown in community gardens in Europe.
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DOI：
10.1016/j.fawpar.2022.e00158
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发表时间：
2022-06
影响因子：
2.5
通讯作者：
Betson, Martha
中科院分区：
文献类型：
--
作者：
Healy, Sara R.;Morgan, Eric R.;Prada, Joaquin M.;Betson, Martha
犬弓形虫和猫弓形虫是狗、猫和狐狸的人畜共患蛔虫。这些确定的宿主通过它们的粪便传递卵子，这些粪便污染环境，随后可能通过土壤或受污染的蔬菜被摄入。在人类中，感染弓形虫可能会对健康造成严重影响。这项概念验证研究旨在调查弓形虫的存在。来自英格兰南部社区菜园的即食蔬菜(生菜、菠菜、葱和芹菜)上的鸡蛋。在英国，之前从未调查过蔬菜受弓形虫卵污染的情况，更广泛地说，这是欧洲社区菜园种植的蔬菜第一次被评估是否受弓形虫卵污染。16个社区花园参与了这项研究，提供了82个适合分析的蔬菜样本。研究参与者还完成了一份匿名问卷，内容是观察到弓形虫确定宿主对这些地点的访问。用添加了一系列弓形虫的生菜样品对鸡蛋回收方法进行了比较。鸡蛋浓缩，沉淀法和离心法提取的鸡蛋数最多。从参与的社区花园中获得的每种蔬菜类型的样本(100微克)都使用优化的方法进行了弓形虫卵的检测。两个生菜样本的弓形虫检测呈阳性。鸡蛋，蔬菜样本的患病率为2.4%(95%CI=1.3-3.5%)，生菜的患病率为6.5%(95%CI=94.7%-8.3%；n=0.31)。问卷数据显示，在研究中，狐狸、猫和狗经常访问社区花园，88%(68/77)的受访者报告说在他们的地点看到了确定的宿主物种或确定宿主的粪便。这项概念验证研究首次显示了弓形虫的存在。在英国种植的蔬菜上以及这些蔬菜起源的土壤中都有鸡蛋，并突出了社区花园中的生物安全和人畜共患病风险。本研究建立了一种评价弓形虫的方法。蔬菜上的虫卵，并为更大规模的弓形虫调查铺平了道路。大田蔬菜上的鸡蛋污染。欧洲首次报道在社区菜园种植的蔬菜上有弓形虫卵。在这些蔬菜的原产地土壤中也检测到了弓形虫。犬弓形虫和猫弓形虫的最终宿主经常造访社区花园。沉淀法从添加的生菜样品中回收的弓形虫虫卵最多。
Toxocara canis and T. cati are zoonotic roundworm parasites of dogs, cats and foxes. These definitive hosts pass eggs in their faeces, which contaminate the environment and can subsequently be ingested via soil or contaminated vegetables. In humans, infection with Toxocara can have serious health implications. This proof-of-concept study aimed to investigate the presence of Toxocara spp. eggs on ‘ready-to-eat’ vegetables (lettuce, spinach, spring onion and celery) sampled from community gardens in southern England. The contamination of vegetables with Toxocara eggs has never been investigated in the UK before, and more widely, this is the first time vegetables grown in community gardens in Europe have been assessed for Toxocara egg contamination. Sixteen community gardens participated in the study, providing 82 vegetable samples fit for analysis. Study participants also completed an anonymous questionnaire on observed visits to the sites by definitive hosts of Toxocara. Comparison of egg recovery methods was performed using lettuce samples spiked with a series of Toxocara spp. egg concentrations, with sedimentation and centrifugal concentration retrieving the highest number of eggs. A sample (100 g) of each vegetable type obtained from participating community gardens was tested for the presence of Toxocara eggs using the optimised method. Two lettuce samples tested positive for Toxocara spp. eggs, giving a prevalence of 2.4% (95% CI =1.3–3.5%) for vegetable samples overall, and 6.5% (95% CI = 4.7–8.3%; n = 31) specifically for lettuce. Questionnaire data revealed that foxes, cats and dogs frequently visited the community gardens in the study, with 88% (68/77) of respondents reporting seeing a definitive host species or the faeces of a definitive host at their site. This proof-of-concept study showed for the first time the presence of Toxocara spp. eggs on vegetables grown in the UK, as well as within the soil where these vegetables originated, and highlights biosecurity and zoonotic risks in community gardens. This study establishes a method for assessment of Toxocara spp. eggs on vegetable produce and paves the way for larger-scale investigations of Toxocara spp. egg contamination on field-grown vegetables. First report of Toxocara eggs on vegetables grown in community gardens in Europe. Toxocara also detected in the soil where these vegetables originated. Definitive hosts for Toxocara canis and T. cati frequently visit community gardens. Sedimentation method recovered the most Toxocara eggs from spiked lettuce samples.
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影响因子：
1.9
作者：
Malkamaki, Sanna;Nareaho, Anu;Sukura, Antti
通讯作者：
Sukura, Antti
影响因子：
6
作者：
Kozan, E;Gonenc, B;Aycicek, H
通讯作者：
Aycicek, H
影响因子：
6
作者：
Chalmers, Rachel M.;Katzer, Frank;Mayer-Scholl, Anne
通讯作者：
Mayer-Scholl, Anne
DOI：
10.1051/parasite/2017031
发表时间：
2017
期刊：
Parasite (Paris, France)
影响因子：
--
作者：
Poulle ML;Bastien M;Richard Y;Josse-Dupuis É;Aubert D;Villena I;Knapp J
通讯作者：
Knapp J
影响因子：
2.6
作者：
Morgan, E. R.;Azam, D.;Pegler, K.
通讯作者：
Pegler, K.