Contraceptive rings promote vaginal lactobacilli in a high bacterial vaginosis prevalence population: A randomised, open-label longitudinal study in Rwandan women.
Contraceptive rings promote vaginal lactobacilli in a high bacterial vaginosis prevalence population: A randomised, open-label longitudinal study in Rwandan women.
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DOI：
10.1371/journal.pone.0201003
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发表时间：
2018
期刊：
影响因子：
3.7
通讯作者：
Ring Plus study group
中科院分区：
文献类型：
--
作者：
Crucitti T;Hardy L;van de Wijgert J;Agaba S;Buyze J;Kestelyn E;Delvaux T;Mwambarangwe L;De Baetselier I;Jespers V;Ring Plus study group
激素避孕与阴道生态失调风险降低有关，而阴道生态失调又与包括艾滋病毒在内的性传播感染（STI）患病率降低有关。阴道环被用作或开发为用于预防或治疗性传播感染和艾滋病毒的避孕激素和抗菌药物的输送系统。我们假设，避孕阴道环（CVR）含有雌激素增强乳酸杆菌为主的阴道微生物群落，尽管CVR的表面上的生物量积累。我们在卢旺达基加利的Rinda Utagima进行了一项开放标签NuvaRing®研究，招募了120名女性，为期12周。通过革兰氏染色Nugent评分（仅阴道）、乳杆菌属、阴道加德纳菌和阴道变形菌的定量PCR以及荧光原位杂交（以观察细胞粘附细菌），在基线和每次环移除访视时评估阴道和环微生物群。通过结晶紫染色测量环生物量。基线时细菌性阴道病（BV）患病率为48%。平均Nugent评分随着瓣膜成型环的使用而显著降低。阴道分泌物中乳酸杆菌的存在和平均log 10浓度显著增加，而G.迷走神经和A.阴道明显缩小。在CVR上积累的生物量具有反映阴道微生物群的物种组成。结晶紫染色后，该环生物质组成和光密度并未随着时间的推移发生显着变化。NuvaRing®在基线BV患病率高的人群中促进了乳酸杆菌占主导地位的阴道微生物群落，尽管环上积累了生物量。
Hormonal contraception has been associated with a reduced risk of vaginal dysbiosis, which in turn has been associated with reduced prevalence of sexually transmitted infections (STIs), including HIV. Vaginal rings are used or developed as delivery systems for contraceptive hormones and antimicrobial drugs for STI and HIV prevention or treatment. We hypothesized that a contraceptive vaginal ring (CVR) containing oestrogen enhances a lactobacilli-dominated vaginal microbial community despite biomass accumulation on the CVR’s surface. We enrolled 120 women for 12 weeks in an open-label NuvaRing® study at Rinda Ubuzima, Kigali, Rwanda. Vaginal and ring microbiota were assessed at baseline and each ring removal visit by Gram stain Nugent scoring (vaginal only), quantitative PCR for Lactobacillus species, Gardnerella vaginalis and Atopobium vaginae, and fluorescent in situ hybridization to visualize cell-adherent bacteria. Ring biomass was measured by crystal violet staining. Bacterial vaginosis (BV) prevalence was 48% at baseline. The mean Nugent score decreased significantly with ring use. The presence and mean log10 concentrations of Lactobacillus species in vaginal secretions increased significantly whereas those of G. vaginalis and presence of A. vaginae decreased significantly. Biomass accumulated on the CVRs with a species composition mirroring the vaginal microbiota. This ring biomass composition and optical density after crystal violet staining did not change significantly over time. NuvaRing® promoted lactobacilli-dominated vaginal microbial communities in a population with high baseline BV prevalence despite the fact that biomass accumulated on the rings.
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DOI：
10.1086/657320
发表时间：
2010-12-15
期刊：
The Journal of infectious diseases
影响因子：
--
作者：
Brotman RM;Klebanoff MA;Nansel TR;Yu KF;Andrews WW;Zhang J;Schwebke JR
通讯作者：
Schwebke JR
影响因子：
17.1
作者：
Gajer P;Brotman RM;Bai G;Sakamoto J;Schütte UM;Zhong X;Koenig SS;Fu L;Ma ZS;Zhou X;Abdo Z;Forney LJ;Ravel J
通讯作者：
Ravel J
DOI：
10.1111/j.1749-6632.2011.06041.x
发表时间：
2011-01-01
期刊：
EVOLUTION OF INFECTIOUS AGENTS IN RELATION TO SEX
影响因子：
--
作者：
Danielsson, Dan;Teigen, Per Kristen;Moi, Harald
通讯作者：
Moi, Harald
影响因子：
5
作者：
Lokken, Erica M.;Balkus, Jennifer E.;Manhart, Lisa E.
通讯作者：
Manhart, Lisa E.
影响因子：
5.2
作者：
Keller, M. J.;Malone, A. M.;Herold, B. C.
通讯作者：
Herold, B. C.