Identification of a Novel Salt Tolerance-Related Locus in Wild Soybean (Glycine soja Sieb. & Zucc.).

Identification of a Novel Salt Tolerance-Related Locus in Wild Soybean (Glycine soja Sieb. & Zucc.).
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DOI:
10.3389/fpls.2021.791175
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发表时间:
2021
影响因子:
5.6
通讯作者:
Qiu L
Qiu L
中科院分区:
生物学2区
文献类型:
--
作者:
Guo X;Jiang J;Liu Y;Yu L;Chang R;Guan R;Qiu L

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盐胁迫是影响大豆生长发育和产量的重要非生物胁迫因子。NY 36 -87是一份高耐盐野生大豆种质。本研究利用来自NY 36 -87的两个F2:3作图群体和两个盐敏感大豆品种中黄39和北京的耐盐相关基因进行了定位。这两个群体的分离比例为1(耐受):2(杂合):1(敏感),符合孟德尔分离模式。利用简单重复序列(SSR)标记结合群体分离分析(BSA)定位策略,在中黄39 × NY 36 -87的F2:3群体03号染色体上定位了一个耐盐基因座,该基因座与已知基因GmSALT 3共分离。在Peking× NY 36 -87的F2:3群体中,检测到了显性耐盐相关基因,并将其定位在第18号染色体上。我们将该基因命名为GmSALT 18,并将其精细定位到241-kb区域。时间进程分析和嫁接实验证实,北京积累更多的Na+在地上部通过根为基础的机制。这些结果表明,耐盐野生大豆品系NY 36 -87中含有耐盐相关基因GmSALT 3和GmSALT 18,为大豆耐盐育种提供了遗传材料和新的位点。
Salinity is an important abiotic stress factor that affects growth and yield of soybean. NY36-87 is a wild soybean germplasm with high salt tolerance. In this study, two F2:3 mapping populations derived from NY36-87 and two salt-sensitive soybean cultivars, Zhonghuang39 and Peking, were used to map salt tolerance-related genes. The two populations segregated as 1 (tolerant):2 (heterozygous):1 (sensitive), indicating a Mendelian segregation model. Using simple sequence repeat (SSR) markers together with the bulked segregant analysis (BSA) mapping strategy, we mapped a salt tolerance locus on chromosome 03 in F2:3 population Zhonghuang39×NY36-87 to a 98-kb interval, in which the known gene GmSALT3 co-segregated with the salt tolerance locus. In the F2:3 population of Peking×NY36-87, the dominant salt tolerance-associated gene was detected and mapped on chromosome 18. We named this gene GmSALT18 and fine mapped it to a 241-kb region. Time course analysis and a grafting experiment confirmed that Peking accumulated more Na+ in the shoot via a root-based mechanism. These findings reveal that the tolerant wild soybean line NY36-87 contains salt tolerance-related genes GmSALT3 and GmSALT18, providing genetic material and a novel locus for breeding salt-tolerant soybean.
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